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West Nile Virus in Culex Mosquitoes in Central Macedonia, Greece, 2022.
Tsioka, Katerina; Gewehr, Sandra; Pappa, Styliani; Kalaitzopoulou, Stella; Stoikou, Konstantina; Mourelatos, Spiros; Papa, Anna.
Afiliación
  • Tsioka K; Laboratory of Microbiology, National Reference Centre for Arboviruses, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
  • Gewehr S; Ecodevelopment S.A., 57010 Thessaloniki, Greece.
  • Pappa S; Laboratory of Microbiology, National Reference Centre for Arboviruses, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
  • Kalaitzopoulou S; Ecodevelopment S.A., 57010 Thessaloniki, Greece.
  • Stoikou K; Laboratory of Microbiology, National Reference Centre for Arboviruses, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
  • Mourelatos S; Ecodevelopment S.A., 57010 Thessaloniki, Greece.
  • Papa A; Laboratory of Microbiology, National Reference Centre for Arboviruses, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Viruses ; 15(1)2023 01 13.
Article en En | MEDLINE | ID: mdl-36680264
ABSTRACT
In 2022, Greece was the second most seriously affected European country in terms of the West Nile virus (WNV), after Italy. Specifically, Central Macedonia was the region with the most reported human cases (81.5%). In the present study, 30,816 female Culex pipiens sensu lato mosquitoes were collected from May to September 2022 in the seven regional units of Central Macedonia; they were then grouped into 690 pools and tested for WNV, while next-generation sequencing was applied to the samples, which showed a cycle threshold of Ct < 30 in a real-time RT-PCR test. WNV was detected in 5.9% of pools, with significant differences in the detection rate among regional units and months. It is of interest that in the Thessaloniki regional unit, where most of the human cases were observed, the virus circulation started earlier, peaked earlier, and lasted longer than in the other regional units. All sequences clustered into the Central European subclade of WNV lineage 2, and the virus strain differed from the initial Greek strain of 2010 by 0.52% and 0.27% at the nucleotide and amino acid levels, respectively. Signature substitutions were present, such as S73P and T157A in the prM and E structural proteins, respectively. The screening of mosquitoes provides useful information for virus circulation in a region with a potential for early warning, while the availability of whole-genome sequences is essential for further studies, including virus evolution.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fiebre del Nilo Occidental / Virus del Nilo Occidental / Culex / Culicidae Límite: Animals / Female / Humans País/Región como asunto: Europa Idioma: En Revista: Viruses Año: 2023 Tipo del documento: Article País de afiliación: Grecia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fiebre del Nilo Occidental / Virus del Nilo Occidental / Culex / Culicidae Límite: Animals / Female / Humans País/Región como asunto: Europa Idioma: En Revista: Viruses Año: 2023 Tipo del documento: Article País de afiliación: Grecia