T-independent responses to polysaccharides in humans mobilize marginal zone B cells prediversified against gut bacterial antigens.
Sci Immunol
; 8(79): eade1413, 2023 01 27.
Article
en En
| MEDLINE
| ID: mdl-36706172
ABSTRACT
Marginal zone (MZ) B cells are one of the main actors of T-independent (TI) responses in mice. To identify the B cell subset(s) involved in such responses in humans, we vaccinated healthy individuals with Pneumovax, a model TI vaccine. By high-throughput repertoire sequencing of plasma cells (PCs) isolated 7 days after vaccination and of different B cell subpopulations before and after vaccination, we show that the PC response mobilizes large clones systematically, including an immunoglobulin M component, whose diversification and amplification predated the pneumococcal vaccination. These clones could be mainly traced back to MZ B cells, together with clonally related IgA+ and, to a lesser extent, IgG+CD27+ B cells. Recombinant monoclonal antibodies isolated from large PC clones recognized a wide array of bacterial species from the gut flora, indicating that TI responses in humans largely mobilize MZ and switched B cells that most likely prediversified during mucosal immune responses against bacterial antigens and acquired pneumococcal cross-reactivity through somatic hypermutation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Subgrupos de Linfocitos B
/
Antígenos Bacterianos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Sci Immunol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Francia