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Genomics and biochemical analyses reveal a metabolon key to ß-L-ODAP biosynthesis in Lathyrus sativus.
Edwards, Anne; Njaci, Isaac; Sarkar, Abhimanyu; Jiang, Zhouqian; Kaithakottil, Gemy George; Moore, Christopher; Cheema, Jitender; Stevenson, Clare E M; Rejzek, Martin; Novák, Petr; Vigouroux, Marielle; Vickers, Martin; Wouters, Roland H M; Paajanen, Pirita; Steuernagel, Burkhard; Moore, Jonathan D; Higgins, Janet; Swarbreck, David; Martens, Stefan; Kim, Colin Y; Weng, Jing-Ke; Mundree, Sagadevan; Kilian, Benjamin; Kumar, Shiv; Loose, Matt; Yant, Levi; Macas, Jirí; Wang, Trevor L; Martin, Cathie; Emmrich, Peter M F.
Afiliación
  • Edwards A; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Njaci I; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Sarkar A; Biosciences eastern and central Africa International Livestock Research Institute Hub, ILRI campus, Naivasha Road, P.O. 30709, Nairobi, 00100, Kenya.
  • Jiang Z; Queensland University of Technology, 2 George St, Brisbane City, QLD, 4000, Australia.
  • Kaithakottil GG; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Moore C; National Institute of Agricultural Botany, 93 Laurence Weaver Road, Cambridge, CB3 0LE, UK.
  • Cheema J; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Stevenson CEM; School of Traditional Chinese Medicine, Capital Medical University, You An Men, Beijing, 100069, PR China.
  • Rejzek M; Earlham Institute, Norwich Research Park, Colney Lane, Norwich, NR4 7UZ, UK.
  • Novák P; School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
  • Vigouroux M; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Vickers M; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Wouters RHM; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Paajanen P; Institute of Plant Molecular Biology, Biology Centre CAS, Branisovska 31, Ceske Budejovice, CZ-37005, Czech Republic.
  • Steuernagel B; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Moore JD; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Higgins J; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Swarbreck D; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Martens S; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Kim CY; John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
  • Weng JK; Earlham Institute, Norwich Research Park, Colney Lane, Norwich, NR4 7UZ, UK.
  • Mundree S; Earlham Institute, Norwich Research Park, Colney Lane, Norwich, NR4 7UZ, UK.
  • Kilian B; Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38098, San Michele all' Adige (TN), Italy.
  • Kumar S; Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, USA.
  • Loose M; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Yant L; Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, USA.
  • Macas J; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Wang TL; Queensland University of Technology, 2 George St, Brisbane City, QLD, 4000, Australia.
  • Martin C; Global Crop Diversity Trust, Platz der Vereinten Nationen 7, 53113, Bonn, Germany.
  • Emmrich PMF; International Center for Agricultural Research in the Dry Areas, Avenue Hafiane Cherkaoui, Rabat, Morocco.
Nat Commun ; 14(1): 876, 2023 02 16.
Article en En | MEDLINE | ID: mdl-36797319
Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop's association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, ß-L-oxalyl-2,3-diaminopropionic acid (ß-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce ß-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lathyrus / Aminoácidos Diaminos Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lathyrus / Aminoácidos Diaminos Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido