Altered hormonal responses: markers for embryonal and embryonic carcinoma stem cells and their differentiated derivatives.
J Cell Physiol
; 133(2): 405-8, 1987 Nov.
Article
en En
| MEDLINE
| ID: mdl-3680397
ABSTRACT
Teratocarcinoma cells in culture offer an in vitro system for studying certain aspects of embryonic differentiation. To gain some insight into regulatory systems that might be operative during early development, we have characterized the alterations that occur in the hormonal responsiveness of the membrane adenylate cyclase of different embryonal carcinoma cell lines with differentiation. Each undifferentiated embryonal carcinoma stem cell studied (F9, PCC4, PC13, P19) has an adenylate cyclase system predominantly activated by calcitonin. Of great interest is the fact that cAMP production is also enhanced specifically by calcitonin in an embryo-derived stem cell line. Differentiation of the embryonal carcinoma stem cell population toward parietal endoderm results in a decrease in calcitonin activation with a concomitant appearance of sensitivity to parathyroid hormone. Differentiation toward visceral endoderm is characterized by a lack of response of the adenylate cyclase system to both calcitonin and parathyroid hormone. These results indicate that the changes noted in adenylate cyclase hormonal responsiveness might serve as useful markers during early stages of differentiation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Teratoma
/
Células Madre Neoplásicas
/
Biomarcadores de Tumor
/
Adenilil Ciclasas
/
Hormonas
Límite:
Animals
Idioma:
En
Revista:
J Cell Physiol
Año:
1987
Tipo del documento:
Article
País de afiliación:
Francia