Effects of Ca2+ ions on the horseshoe crab coagulation cascade triggered by lipopolysaccharide.

Yamashita, Keisuke; Takahashi, Daisuke; Yamamoto, Yuki; Kiyomoto, Shingo; Shibata, Toshio; Kawabata, Shun-Ichiro

Yamashita, Keisuke; Takahashi, Daisuke; Yamamoto, Yuki; Kiyomoto, Shingo; Shibata, Toshio; Kawabata, Shun-Ichiro.

Afiliación

Yamashita K; Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395.
Takahashi D; Faculty of Pharmaceutical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582.
Yamamoto Y; Daisuke Takahashi, Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Nishi-ku, Kumamoto 860-0082, Japan.
Kiyomoto S; Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395.
Shibata T; Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395.
Kawabata SI; Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395.

J Biochem ; 174(1): 47-58, 2023 Jun 30.

Article en En | MEDLINE | ID: mdl-36805939

Asunto(s)

Cangrejos Herradura; Lipopolisacáridos; Animales; Lipopolisacáridos/metabolismo; Calcio/metabolismo; Coagulación Sanguínea; Precursores Enzimáticos/metabolismo

Palabras clave

calcium ionsAbbreviations: AMC, 7-amino-4-methylcoumarin; Boc, t-butoxycarbonyl; CBB, Coomassie Brilliant Blue; ce, clotting enzyme; EDTA, ethylenediaminetetraacetic acid; GnTI, N-acetylglucosaminyltransferase I; HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; HSA, human serum albumin; ITC, isothermal titration calorimetry; LPS, lipopolysaccharide; MCA, 4-methylcoumaryl-7-amide; proB, prochelicerase B; proC, prochelicerase C; proCE, proclotting enzyme; proG, prochelicerase G; SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis; SEM, standard error of the mean; WT, wild-type; horseshoe crab hemolymph coagulation; lipopolysaccharide; recombinant protein; serine protease

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lipopolisacáridos / Cangrejos Herradura Límite: Animals Idioma: En Revista: J Biochem Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido

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