Expanding the Toolbox for Functional Genomics in <i>Fonsecaea pedrosoi</i>: The Use of Split-Marker and Biolistic Transformation for Inactivation of Tryptophan Synthase (<i>trpB</i>) Gene.

Favilla, Luísa Dan; Herman, Tatiana Sobianski; Goersch, Camila da Silva; de Andrade, Rosangela Vieira; Felipe, Maria Sueli Soares; Bocca, Anamélia Lorenzetti; Fernandes, Larissa

Expanding the Toolbox for Functional Genomics in Fonsecaea pedrosoi: The Use of Split-Marker and Biolistic Transformation for Inactivation of Tryptophan Synthase (trpB) Gene.

Favilla, Luísa Dan; Herman, Tatiana Sobianski; Goersch, Camila da Silva; de Andrade, Rosangela Vieira; Felipe, Maria Sueli Soares; Bocca, Anamélia Lorenzetti; Fernandes, Larissa.

Afiliación

Favilla LD; Laboratory of Applied Immunology, Institute of Biology, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Federal District, Brasilia 70910-900, Brazil.
Herman TS; Graduate Program in Molecular Biology, Institute of Biology, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Federal District, Brasilia 70910-900, Brazil.
Goersch CDS; Laboratory of Applied Immunology, Institute of Biology, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Federal District, Brasilia 70910-900, Brazil.
de Andrade RV; Graduate Program in Molecular Patology, Faculty of Medicine, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Federal District, Brasilia 70910-900, Brazil.
Felipe MSS; Laboratory of Applied Immunology, Institute of Biology, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Federal District, Brasilia 70910-900, Brazil.
Bocca AL; Graduate Program in Microbial Biology, Institute of Biology, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Federal District, Brasilia 70910-900, Brazil.
Fernandes L; Graduate Program of Genomic Sciences and Biotechnology, Catholic University of Brasilia, Campus Asa Norte, Asa Norte, Federal District, Taguatinga 70790-160, Brazil.

J Fungi (Basel) ; 9(2)2023 Feb 08.

Article en En | MEDLINE | ID: mdl-36836338

Palabras clave

Fonsecaea; biolistic transformation; chromoblastomycosis; double-joint PCR; gene replacement by homologous recombination; knockout mutant; trpB; tryptophan biosynthesis

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza

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