Preparation of chaperone-loaded neural stem cell-derived extracellular vesicles to reduce protein aggregation in Huntington's disease cellular models.
STAR Protoc
; 4(1): 102134, 2023 03 17.
Article
en En
| MEDLINE
| ID: mdl-36861837
ABSTRACT
Here, we present a protocol using genetic engineering techniques to prepare small extracellular vesicles (sEVs) enriched in the chaperone protein DNAJB6. We describe steps to prepare cell lines overexpressing DNAJB6, followed by the isolation and characterization of sEVs from cell conditioned media. Further, we describe assays to examine effects of DNAJB6-loaded sEVs on protein aggregation in Huntington's disease cellular models. The protocol can be readily repurposed to study protein aggregation in other neurodegenerative disorders or extended to other therapeutic proteins. For complete details on the use and execution of this protocol, please refer to Joshi et al. (2021).1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedad de Huntington
/
Enfermedades Neurodegenerativas
/
Células-Madre Neurales
/
Vesículas Extracelulares
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article