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Molecular acclimation of Halobacterium salinarum to halite brine inclusions.
Favreau, Charly; Tribondeau, Alicia; Marugan, Marie; Guyot, François; Alpha-Bazin, Beatrice; Marie, Arul; Puppo, Remy; Dufour, Thierry; Huguet, Arnaud; Zirah, Séverine; Kish, Adrienne.
Afiliación
  • Favreau C; Unité Molécules de Communication et Adaptation des Microorganismes (MCAM), Muséum National d'Histoire Naturelle (MNHN), CNRS, Paris, France.
  • Tribondeau A; Unité Physiologie Moléculaire et Adaptation (PhyMA), MNHN, CNRS, Paris, France.
  • Marugan M; Unité Molécules de Communication et Adaptation des Microorganismes (MCAM), Muséum National d'Histoire Naturelle (MNHN), CNRS, Paris, France.
  • Guyot F; Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie (IMPMC), MNHN, Sorbonne Université, CNRS, IRD, Paris, France.
  • Alpha-Bazin B; Département Médicaments et Technologies pour la Santé (DMTS), Université Paris-Saclay, CEA, INRAE, SPI, Bagnols-sur-Cèze, France.
  • Marie A; Unité Molécules de Communication et Adaptation des Microorganismes (MCAM), Muséum National d'Histoire Naturelle (MNHN), CNRS, Paris, France.
  • Puppo R; Unité Molécules de Communication et Adaptation des Microorganismes (MCAM), Muséum National d'Histoire Naturelle (MNHN), CNRS, Paris, France.
  • Dufour T; Laboratoire de Physique des Plasma (LPP), Sorbonne Université, CNRS, École Polytechnique, Université Paris-Sud, Observatoire de Paris, Paris, France.
  • Huguet A; Unité Milieux Environnementaux Transferts et Interactions dans les hydrosystèmes et les Sols (METIS), Sorbonne Université, CNRS, EPHE, PSL, Paris, France.
  • Zirah S; Unité Molécules de Communication et Adaptation des Microorganismes (MCAM), Muséum National d'Histoire Naturelle (MNHN), CNRS, Paris, France.
  • Kish A; Unité Molécules de Communication et Adaptation des Microorganismes (MCAM), Muséum National d'Histoire Naturelle (MNHN), CNRS, Paris, France.
Front Microbiol ; 13: 1075274, 2022.
Article en En | MEDLINE | ID: mdl-36875534
Halophilic microorganisms have long been known to survive within the brine inclusions of salt crystals, as evidenced by the change in color for salt crystals containing pigmented halophiles. However, the molecular mechanisms allowing this survival has remained an open question for decades. While protocols for the surface sterilization of halite (NaCl) have enabled isolation of cells and DNA from within halite brine inclusions, "-omics" based approaches have faced two main technical challenges: (1) removal of all contaminating organic biomolecules (including proteins) from halite surfaces, and (2) performing selective biomolecule extractions directly from cells contained within halite brine inclusions with sufficient speed to avoid modifications in gene expression during extraction. In this study, we tested different methods to resolve these two technical challenges. Following this method development, we then applied the optimized methods to perform the first examination of the early acclimation of a model haloarchaeon (Halobacterium salinarum NRC-1) to halite brine inclusions. Examinations of the proteome of Halobacterium cells two months post-evaporation revealed a high degree of similarity with stationary phase liquid cultures, but with a sharp down-regulation of ribosomal proteins. While proteins for central metabolism were part of the shared proteome between liquid cultures and halite brine inclusions, proteins involved in cell mobility (archaellum, gas vesicles) were either absent or less abundant in halite samples. Proteins unique to cells within brine inclusions included transporters, suggesting modified interactions between cells and the surrounding brine inclusion microenvironment. The methods and hypotheses presented here enable future studies of the survival of halophiles in both culture model and natural halite systems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza