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Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish.
Drake, Louisa K; Keatinge, Marcus; Tsarouchas, Themistoklis M; Becker, Catherina G; Lyons, David A; Becker, Thomas.
Afiliación
  • Drake LK; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK.
  • Keatinge M; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK.
  • Tsarouchas TM; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK.
  • Becker CG; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK.
  • Lyons DA; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
  • Becker T; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol ; 2636: 263-277, 2023.
Article en En | MEDLINE | ID: mdl-36881306
ABSTRACT
Larval zebrafish show axonal regrowth over a complex spinal injury site and recovery of function within days after injury. Here we describe a simple protocol to disrupt gene function in this model using acute injections of highly active synthetic gRNAs to rapidly detect loss-of-function phenotypes without the need for breeding.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Pez Cebra Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Pez Cebra Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido