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Quantitative Polymerase Chain Reaction from Malaria Rapid Diagnostic Tests to Detect Borrelia crocidurae, the Agent of Tick-Borne Relapsing Fever, in Febrile Patients in Senegal.
El Hadji Ibrahima, Ndiaye; Diatta, Georges; Adama Zan, Diarra; Bassene, Hubert; Sokhna, Cheikh; Parola, Philippe.
Afiliación
  • El Hadji Ibrahima N; Aix Marseille Univ, Institut de Recherche pour le Développement (IRD), APHM, SSA, Vecteurs-Infections Tropicales et Méditerranéennes (VITROME), Marseille, France.
  • Diatta G; Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Marseille, France.
  • Adama Zan D; VITROME, IRD UMR 257, Campus International IRD-UCAD Hann, Dakar, Sénégal.
  • Bassene H; VITROME, IRD UMR 257, Campus International IRD-UCAD Hann, Dakar, Sénégal.
  • Sokhna C; Aix Marseille Univ, Institut de Recherche pour le Développement (IRD), APHM, SSA, Vecteurs-Infections Tropicales et Méditerranéennes (VITROME), Marseille, France.
  • Parola P; Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Marseille, France.
Am J Trop Med Hyg ; 108(5): 968-976, 2023 05 03.
Article en En | MEDLINE | ID: mdl-36913922
ABSTRACT
In endemic malaria areas, Plasmodium is currently diagnosed mainly through the use of rapid diagnostic tests (RDTs). However, in Senegal, many causes of fever remain unknown. Tick-borne relapsing fever, an often-neglected public health problem, is the main cause of consultation for acute febrile illness after malaria and flu in rural areas. Our objective was to test the feasibility of extracting and amplifying DNA fragments by quantitative polymerase chain reaction (qPCR) from malaria-negative RDTs for Plasmodium falciparum (malaria Neg RDTs P.f) to detect Borrelia spp. and other bacteria. Between January and December 2019, malaria Neg RDTs P.f were collected on a quarterly basis in 12 health facilities in four regions of Senegal. The DNA extracted from the malaria Neg RDTs P.f was tested using qPCR and the results were confirmed by standard PCR and sequencing. Only Borrelia crocidurae DNA was detected in 7.22% (159/2,202) of RDTs. The prevalence of B. crocidurae DNA was higher in July (16.47%, 43/261) and August (11.21%, 50/446). The annual prevalence was 9.2% (47/512) and 5.0% (12/241) in Ngayokhem and Nema-Nding, respectively, health facilities in the Fatick region. Our study confirms that B. crocidurae infection is a frequent cause of fever in Senegal, with a high prevalence of cases in health facilities in the regions of Fatick and Kaffrine. Malaria Neg RDTs P.f are potentially a good source of pathogen sampling for the molecular identification of other causes of fever of unknown origin, even in the most remote areas.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fiebre Recurrente / Borrelia / Malaria Falciparum / Malaria Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Límite: Humans País/Región como asunto: Africa Idioma: En Revista: Am J Trop Med Hyg Año: 2023 Tipo del documento: Article País de afiliación: Francia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fiebre Recurrente / Borrelia / Malaria Falciparum / Malaria Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Límite: Humans País/Región como asunto: Africa Idioma: En Revista: Am J Trop Med Hyg Año: 2023 Tipo del documento: Article País de afiliación: Francia