Arbuscular mycorrhiza fungus alleviates arsenic mediated disturbances in tricarboxylic acid cycle and nitrogen metabolism in Triticum aestivum L.
Plant Physiol Biochem
; 197: 107631, 2023 Apr.
Article
en En
| MEDLINE
| ID: mdl-36965318
ABSTRACT
Utilization of arbuscular mycorrhizal (AM) fungi (AMF) as a sustainable strategy in redeeming arsenic (As) toxicity in plants is a promising approach. Low As accumulation, restoration of physiological processes, and As tolerance by AMF have been documented in crop plants. However, to comprehend AM-mediated As tolerance in plants, understanding the biochemical responses of host to the symbiont is crucial. The study evaluated the effect of an AM fungus, Rhizophagus intraradices on tricarboxylic acid cycle (TCA) and nitrogen metabolism of Triticum aestivum under three As concentrations (0, 25, and 50 mg As kg-1 soil). Results showed that TCA cycle and nitrogen metabolism were severely impaired by As that resulted into a higher C/N ratio. However, colonization by R. intraradices attenuated As mediated alterations in TCA cycle by augmenting the activity of pyruvate dehydrogenase that provided sufficient substrate for the TCA cycle. Furthermore, mycorrhizal (M) plants reinstated the activities of isocitrate dehydrogenase, succinate dehydrogenase, fumarase, and malate dehydrogenase even under high As level. Although citrate synthase and oxoglutarate dehydrogenase activities declined upon As exposure in M-plants, these were nevertheless higher than their non-mycorrhizal (NM) counterparts, ensuring higher levels of citric acid and succinic acid in M-plants. AM colonization also moderated the As-mediated disturbances in nitrogen assimilation by augmenting the activity of nitrate reductase, nitrite reductase, glutamine synthase, and glutamine-2-oxoglutarate amino transferase. Overall findings of the study point out that colonization by R. intraradices favourably regulated the TCA cycle and nitrogen metabolism and confronted As-mediated alterations in C/N ratio.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arsénico
/
Micorrizas
Idioma:
En
Revista:
Plant Physiol Biochem
Asunto de la revista:
BIOQUIMICA
/
BOTANICA
Año:
2023
Tipo del documento:
Article
País de afiliación:
India