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Sperm mRNA screening by reverse transcription-recombinase polymerase amplification (RT-RPA) assay: Decision-making for differential extraction.
Kubo, Seiji; Niimi, Hideki; Kitajima, Isao.
Afiliación
  • Kubo S; Department of Clinical Laboratory and Molecular Pathology, Faculty of Medicine, Academic Assembly, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan; Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters, 1-1 Kuratsuki, Kanazawa, 920-8553, Japan. Electronic address: n2051101@ems.u-toyama.ac.jp.
  • Niimi H; Department of Clinical Laboratory and Molecular Pathology, Faculty of Medicine, Academic Assembly, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan. Electronic address: hiniimi@med.u-toyama.ac.jp.
  • Kitajima I; Administrative Office, University of Toyama, 3190 Gofuku, Toyama, 930-8555, Japan.
Anal Biochem ; 670: 115121, 2023 06 01.
Article en En | MEDLINE | ID: mdl-36972745
The presence of sperm cells is an indicator for differential extraction on sexual assault samples. In general, sperm cells are identified by microscopic analysis; however, this conventional method takes time and effort, even for trained personnel. Here, we present a reverse transcription-recombinase polymerase amplification (RT-RPA) assay targeting sperm mRNA marker (PRM1). The RT-RPA assay requires only 40 min for PRM1 detection and demonstrates a sensitivity of 0.1 µL of semen. Our results indicate that the RT-RPA assay may be a rapid, simple, and specific strategy for screening sperm cells in sexual assault samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Recombinasas / Transcripción Reversa Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans / Male Idioma: En Revista: Anal Biochem Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Recombinasas / Transcripción Reversa Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans / Male Idioma: En Revista: Anal Biochem Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos