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A short-term method to evaluate anti-leishmania drugs by inhibition of stage differentiation in Leishmania mexicana using flow cytometry.
Teh-Poot, Christian Florian; Dzul-Huchim, Victor Manuel; Mercado, Jonathan M; Villanueva-Lizama, Liliana Estefanía; Bottazzi, Maria Elena; Jones, Kathryn M; Tsai, Francis T F; Cruz-Chan, Julio Vladimir.
Afiliación
  • Teh-Poot CF; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
  • Dzul-Huchim VM; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
  • Mercado JM; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
  • Villanueva-Lizama LE; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico; Texas Children's Hospital Center for Vaccine Development, Houston, TX, USA; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medi
  • Bottazzi ME; Texas Children's Hospital Center for Vaccine Development, Houston, TX, USA; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA; Department of Biochemistry and Molecular Biology, and Department of Molecular Virology and Microbiology, Baylor Co
  • Jones KM; Texas Children's Hospital Center for Vaccine Development, Houston, TX, USA; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA; Department of Biochemistry and Molecular Biology, and Department of Molecular Virology and Microbiology, Baylor Co
  • Tsai FTF; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA; Department of Biochemistry and Molecular Biology, and Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, 77030, USA.
  • Cruz-Chan JV; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico; Texas Children's Hospital Center for Vaccine Development, Houston, TX, USA; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medi
Exp Parasitol ; 249: 108519, 2023 Jun.
Article en En | MEDLINE | ID: mdl-37004860
Leishmaniasis is a vector-borne neglected tropical disease caused by the Leishmania spp. Parasite. The disease is transmitted to humans and animals by the bite of infected female sandflies during the ingestion of bloodmeal. Because current drug treatments induce toxicity and parasite resistance, there is an urgent need to evaluate new drugs. Most therapeutics target the differentiation of promastigotes to amastigotes, which is necessary to maintain Leishmania infection. However, in vitro assays are laborious, time-consuming, and depend on the experience of the technician. In this study, we aimed to establish a short-term method to assess the differentiation status of Leishmania mexicana (L. mexicana) using flow cytometry. Here, we showed that flow cytometry provides a rapid means to quantify parasite differentiation in cell culture as reliably as light microscopy. Interestingly, we found using flow cytometry that miltefosine reduced promastigote-to-amastigote differentiation of L. mexicana. We conclude that flow cytometry provides a means to rapidly assay the efficacy of small molecules or natural compounds as potential anti-leishmanials.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmania mexicana / Leishmaniasis / Leishmania Límite: Animals / Female / Humans País/Región como asunto: Mexico Idioma: En Revista: Exp Parasitol Año: 2023 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmania mexicana / Leishmaniasis / Leishmania Límite: Animals / Female / Humans País/Región como asunto: Mexico Idioma: En Revista: Exp Parasitol Año: 2023 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos