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MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma.
Wang, Qi-Wei; Sun, Ya-Nan; Tan, Li-Jun; Zhao, Jian-Nan; Zhou, Xiao-Jie; Yu, Tian-Jiao; Liu, Jiang-Tao.
Afiliación
  • Wang QW; Department of Otolaryngology, Head and Neck Surgery, Harbin Medical University, Harbin, People's Republic of China.
  • Sun YN; Department of Otolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, No.246, Xuefu Road, Harbin, 150081, People's Republic of China. syn2767@126.com.
  • Tan LJ; Department of Oncology, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
  • Zhao JN; Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, People's Republic of China.
  • Zhou XJ; Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, People's Republic of China.
  • Yu TJ; Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, People's Republic of China.
  • Liu JT; Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, People's Republic of China. ljt89103@163.com.
Mol Biol Rep ; 50(6): 5307-5317, 2023 Jun.
Article en En | MEDLINE | ID: mdl-37155009
BACKGROUND: MiRNAs can affect the radiosensitization of head and neck squamous cell carcinoma (HNSCC). We aimed to analyze the function of miR-125 family members in HNSCC using The Cancer Genome Atlas (TCGA) and determine their effect on radiation in laryngeal squamous cell cancer (LSCC). METHODS: First, we systematically analyzed the role of the miR-125 family in HNSCC using the TCGA database and found that miR-125a-5p is associated with radiotherapy. We then performed comprehensive enrichment analysis of miR-125a-5p and predicted target genes. Then, we performed transfection, cell proliferation assays, reverse transcription polymerase chain reaction, apoptosis assays, micronucleus tests, and western blotting on hep-2 cells selected with puromycin. RESULTS: MiR-125 family members exhibited significantly different expression in HNSCC. They were significantly associated with tumor-node-metastasis staging, clinical stages, and histological grades. Radiation therapy had a statistically effect on miR-125 family members, except miR-125a-3p. Moreover, miR-125a-5p was related to overall survival in LSCC. Thus, we predicted 110 target genes and seven hub genes of miR-125a-5p. The proliferation rate of cells transfected with lentivirus vector expressing miR-125a-5p was significantly reduced compared to the other groups. The radiation effect was enhanced in cells transfected with miR-125a-5p. The ratio of apoptotic cells transfected and exposed to X-rays (10 Gy) was distinctly higher than that of the Ad-control group. Western blotting analysis revealed that miR-125a-5p upregulated the apoptotic regulators P53 and rH2AX. Thus, miR-125a-5p may increase radiosensitivity in LSCC via upregulation of pro-apoptotic genes. CONCLUSIONS: MiR-125 family members could be prognostic biomarkers of HNSCC and improve HNSCC sensitivity to radiotherapy by activating P53. Upregulating miR-125a-5p via lentivirus vectors may be a novel strategy to strengthen the effect of radiotherapy on LSCC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Neoplasias de Cabeza y Cuello Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Neoplasias de Cabeza y Cuello Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos