Protein overproduction alters exosome secretion in Chinese hamster ovary cells.
Anal Bioanal Chem
; 415(16): 3167-3176, 2023 Jul.
Article
en En
| MEDLINE
| ID: mdl-37160422
Despite the abundance of available cell lines, nearly 70% of all recombinant therapeutic proteins today are produced in Chinese hamster ovary (CHO) cells. The impact of protein overproduction on the secretion of exosomes by CHO cells has been investigated here. Increased secretion of extracellular vesicles (EVs) by protein overexpressing CHO cells was demonstrated with protein content assay, nanoparticle tracking analysis, and capillary electrophoresis. Our results revealed that a protein overproduction might induce EVs secretion, which might be accompanied by the sequestration and loading of overexpressed proteins into the exosomes. These findings are of vital importance for the manufacturing of therapeutics in CHO expression systems due to the risk of product loss during downstream processing of culture medium as well as the application of exosomes as nanocarriers of therapeutic proteins. The study indicates also the importance of culturing process control.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Exosomas
/
Vesículas Extracelulares
Límite:
Animals
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2023
Tipo del documento:
Article
País de afiliación:
Polonia
Pais de publicación:
Alemania