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Multicentre genetic diversity study of carbapenem-resistant Enterobacterales: predominance of untypeable pUVA-like blaKPC bearing plasmids.
Simner, Patricia J; Bergman, Yehudit; Fan, Yunfan; Jacobs, Emily B; Ramakrishnan, Srividya; Lu, Jennifer; Lewis, Shawna; Hanlon, Ann; Tamma, Pranita D; Schatz, Michael C; Timp, Winston; Carroll, Karen C.
Afiliación
  • Simner PJ; Department of Pathology, Division of Medical Microbiology, Johns Hopkins University School of Medicine, 600 N Wolfe Street, Meyer B1-125, Baltimore, MD, USA.
  • Bergman Y; Department of Medicine, Division of Infectious Disease, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
  • Fan Y; Department of Pathology, Division of Medical Microbiology, Johns Hopkins University School of Medicine, 600 N Wolfe Street, Meyer B1-125, Baltimore, MD, USA.
  • Jacobs EB; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
  • Ramakrishnan S; Department of Pathology, Division of Medical Microbiology, Johns Hopkins University School of Medicine, 600 N Wolfe Street, Meyer B1-125, Baltimore, MD, USA.
  • Lu J; Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.
  • Lewis S; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
  • Hanlon A; Center for Computations Biology, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, USA.
  • Tamma PD; Department of Pathology, Division of Medical Microbiology, Johns Hopkins University School of Medicine, 600 N Wolfe Street, Meyer B1-125, Baltimore, MD, USA.
  • Schatz MC; Department of Pathology, Division of Medical Microbiology, Johns Hopkins University School of Medicine, 600 N Wolfe Street, Meyer B1-125, Baltimore, MD, USA.
  • Timp W; Department of Pediatrics, Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
  • Carroll KC; Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.
JAC Antimicrob Resist ; 5(3): dlad061, 2023 Jun.
Article en En | MEDLINE | ID: mdl-37251303
ABSTRACT

Objectives:

Carbapenem-resistant Enterobacterales (CRE) are an urgent public health threat. A better understanding of the molecular epidemiology and transmission dynamics of CRE is necessary to limit their dissemination within healthcare settings. We sought to investigate the mechanisms of resistance and spread of CRE within multiple hospitals in Maryland.

Methods:

From 2016 to 2018, all CRE were collected from any specimen source from The Johns Hopkins Medical Institutions. The isolates were further characterized using both phenotypic and genotypic approaches, including short- and/or long-read WGS.

Results:

From 2016 to 2018, 302 of 40 908 (0.7%) unique Enterobacterales isolates were identified as CRE. Of CRE, 142 (47%) were carbapenemase-producing CRE with KPC (80.3%) predominating among various genera. Significant genetic diversity was identified among all CRE with high-risk clones serving as major drivers of clonal clusters. Further, we found the predominance of pUVA-like plasmids, with a subset harbouring resistance genes to environmental cleaning agents, involved in intergenus dissemination of blaKPC genes.

Conclusions:

Our findings provide valuable data to understand the transmission dynamics of all CRE within the greater Maryland region. These data can help guide targeted interventions to limit CRE transmission in healthcare facilities.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: JAC Antimicrob Resist Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: JAC Antimicrob Resist Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos