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Limosilactobacillus mucosae-derived extracellular vesicles modulates macrophage phenotype and orchestrates gut homeostasis in a diarrheal piglet model.
Li, Jingjing; Feng, Shuaifei; Wang, Zhenyu; He, Jinhui; Zhang, Zeyue; Zou, Huicong; Wu, Zhifeng; Liu, Xiangdong; Wei, Hong; Tao, Shiyu.
Afiliación
  • Li J; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Feng S; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Wang Z; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.
  • He J; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Zhang Z; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Zou H; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Wu Z; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Liu X; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China. liuxiangdong@mail.hzau.edu.cn.
  • Wei H; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China. weihong63528@163.com.
  • Tao S; College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, China. sytao@mail.hzau.edu.cn.
NPJ Biofilms Microbiomes ; 9(1): 33, 2023 06 06.
Article en En | MEDLINE | ID: mdl-37280255
The diarrheal disease causes high mortality, especially in children and young animals. The gut microbiome is strongly associated with diarrheal disease, and some specific strains of bacteria have demonstrated antidiarrheal effects. However, the antidiarrheal mechanisms of probiotic strains have not been elucidated. Here, we used neonatal piglets as a translational model and found that gut microbiota dysbiosis observed in diarrheal piglets was mainly characterized by a deficiency of Lactobacillus, an abundance of Escherichia coli, and enriched lipopolysaccharide biosynthesis. Limosilactobacillus mucosae and Limosilactobacillus reuteri were a signature bacterium that differentiated healthy and diarrheal piglets. Germ-free (GF) mice transplanted with fecal microbiota from diarrheal piglets reproduced diarrheal disease symptoms. Administration of Limosilactobacillus mucosae but not Limosilactobacillus reuteri alleviated diarrheal disease symptoms induced by fecal microbiota of diarrheal piglets and by ETEC K88 challenge. Notably, Limosilactobacillus mucosae-derived extracellular vesicles alleviated diarrheal disease symptoms caused by ETEC K88 by regulating macrophage phenotypes. Macrophage elimination experiments demonstrated that the extracellular vesicles alleviated diarrheal disease symptoms in a macrophage-dependent manner. Our findings provide insights into the pathogenesis of diarrheal disease from the perspective of intestinal microbiota and the development of probiotic-based antidiarrheal therapeutic strategies.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiota / Antidiarreicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: NPJ Biofilms Microbiomes Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiota / Antidiarreicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: NPJ Biofilms Microbiomes Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos