Identification and functional characterization of developmental-stage-dependent piRNAs in Tibetan sheep testes.
J Anim Sci
; 1012023 Jan 03.
Article
en En
| MEDLINE
| ID: mdl-37282774
The testis in mammals plays an indispensable role in male fertility, in which the development and function are strictly regulated by a variety of non-coding small RNAs. PIWI-interacting RNAs (piRNAs) are the most abundant non-coding small RNAs in mammalian testes, playing an irreplaceable role in testicular development and spermatogenesis. To characterize the piRNA expression and investigate their potential biological function in developmental Tibetan sheep testes, we carried out RNA sequencing. Our results revealed that the length distribution of the identified piRNAs is mostly 2426 nt and 29 nt, exhibiting a preference for uracil at their 5' end and significant ping-pong structure. Most piRNAs were differentially expressed in Tibetan sheep testes in a development-age-dependent manner, and the vast majority of them were upregulated in postpubertal and adult testes. Based on the functional annotation of piRNA target genes, they were mainly implicated in the regulation of gene expression, transcription, protein modification and development during testicular development and spermatogenesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Testículo
/
ARN de Interacción con Piwi
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
J Anim Sci
Año:
2023
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos