A Simple and Flexible Infusion Platform for Automated Native Mass Spectrometry Analysis.
J Am Soc Mass Spectrom
; 34(7): 1528-1531, 2023 Jul 05.
Article
en En
| MEDLINE
| ID: mdl-37291876
ABSTRACT
High throughput native mass spectrometry analysis of proteins and protein complexes has been enabled by recent development of infusion and liquid chromatography (LC) systems, which often include complete LC pumps without fully utilizing their gradient flows. We demonstrated a lower-cost infusion cart for native mass spectrometry applications using a single isocratic solvent pump that can operate at both nano- and high-flow configurations (0.05-150 µL/min) for both infusion and online buffer exchange experiments. The platform is controlled via open-source software and can potentially be expanded for customized experimental designs, offering a lower cost alternative to laboratories with limited budgets and/or needs in student training.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Programas Informáticos
/
Proteínas
Límite:
Humans
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos