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Evaluation of DNA Methylation Profiles of LINE-1, Alu and Ribosomal DNA Repeats in Human Cell Lines Exposed to Radiofrequency Radiation.
Ravaioli, Francesco; Bacalini, Maria Giulia; Giuliani, Cristina; Pellegrini, Camilla; D'Silva, Chiara; De Fanti, Sara; Pirazzini, Chiara; Giorgi, Gianfranco; Del Re, Brunella.
Afiliación
  • Ravaioli F; IRCCS Istituto Delle Scienze Neurologiche di Bologna, 40139 Bologna, Italy.
  • Bacalini MG; IRCCS Istituto Delle Scienze Neurologiche di Bologna, 40139 Bologna, Italy.
  • Giuliani C; Laboratory of Molecular Anthropology and Centre for Genome Biology, Department of Biological, Geological and Environmental Sciences (BIGEA), University of Bologna, 40126 Bologna, Italy.
  • Pellegrini C; IRCCS Istituto Delle Scienze Neurologiche di Bologna, 40139 Bologna, Italy.
  • D'Silva C; IRCCS Istituto Delle Scienze Neurologiche di Bologna, 40139 Bologna, Italy.
  • De Fanti S; IRCCS Istituto Delle Scienze Neurologiche di Bologna, 40139 Bologna, Italy.
  • Pirazzini C; Department of Medical and Surgical Sciences (DIMEC), University of Bologna, 40126 Bologna, Italy.
  • Giorgi G; Department of Pharmacy and Biotechnology (FABIT), University of Bologna, 40126 Bologna, Italy.
  • Del Re B; Department of Pharmacy and Biotechnology (FABIT), University of Bologna, 40126 Bologna, Italy.
Int J Mol Sci ; 24(11)2023 May 27.
Article en En | MEDLINE | ID: mdl-37298336
A large body of evidence indicates that environmental agents can induce alterations in DNA methylation (DNAm) profiles. Radiofrequency electromagnetic fields (RF-EMFs) are radiations emitted by everyday devices, which have been classified as "possibly carcinogenic"; however, their biological effects are unclear. As aberrant DNAm of genomic repetitive elements (REs) may promote genomic instability, here, we sought to determine whether exposure to RF-EMFs could affect DNAm of different classes of REs, such as long interspersed nuclear elements-1 (LINE-1), Alu short interspersed nuclear elements and ribosomal repeats. To this purpose, we analysed DNAm profiles of cervical cancer and neuroblastoma cell lines (HeLa, BE(2)C and SH-SY5Y) exposed to 900 MHz GSM-modulated RF-EMF through an Illumina-based targeted deep bisulfite sequencing approach. Our findings showed that radiofrequency exposure did not affect the DNAm of Alu elements in any of the cell lines analysed. Conversely, it influenced DNAm of LINE-1 and ribosomal repeats in terms of both average profiles and organisation of methylated and unmethylated CpG sites, in different ways in each of the three cell lines studied.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Metilación de ADN / Neuroblastoma Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Metilación de ADN / Neuroblastoma Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza