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Dynamics of antibiotic resistance markers and Escherichia coli invasion in riverine heterotrophic biofilms facing increasing heat and flow stagnation.
Gionchetta, G; Snead, D; Semerad, S; Beck, K; Pruden, A; Bürgmann, H.
Afiliación
  • Gionchetta G; Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Surface Waters - Research and Management, 6047 Kastanienbaum, Switzerland.
  • Snead D; Department of Civil & Environmental Engineering, University of Michigan, Ann Arbor, MI, USA; Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, USA.
  • Semerad S; Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Surface Waters - Research and Management, 6047 Kastanienbaum, Switzerland.
  • Beck K; Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Surface Waters - Research and Management, 6047 Kastanienbaum, Switzerland.
  • Pruden A; Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, USA.
  • Bürgmann H; Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Surface Waters - Research and Management, 6047 Kastanienbaum, Switzerland. Electronic address: helmut.buergmann@eawag.ch.
Sci Total Environ ; 893: 164658, 2023 Oct 01.
Article en En | MEDLINE | ID: mdl-37321511
As motivation to address environmental dissemination of antimicrobial resistance (AMR) is mounting, there is a need to characterize mechanisms by which AMR can propagate under environmental conditions. Here we investigated the effect of temperature and stagnation on the persistence of wastewater-associated antibiotic resistance markers in riverine biofilms and the invasion success of genetically-tagged Escherichia coli. Biofilms grown on glass slides incubated in-situ downstream of a wastewater treatment plant effluent discharge point were transferred to laboratory-scale flumes fed with filtered river water under potentially stressful temperature and flow conditions: recirculation flow at 20 °C, stagnation at 20 °C, and stagnation at 30 °C. After 14 days, quantitative PCR and amplicon sequencing were used to quantify bacteria, biofilms diversity, resistance markers (sul1, sul2, ermB, tetW, tetM, tetB, blaCTX-M-1, intI1) and E. coli. Resistance markers significantly decreased over time regardless of the treatment applied. Although invading E. coli were initially able to colonize the biofilms, its abundance subsequently declined. Stagnation was associated with a shift in biofilm taxonomic composition, but there was no apparent effect of flow conditions or the simulated river-pool warming (30 °C) on AMR persistence or invasion success of E. coli. Results however indicated that antibiotic resistance markers in the riverine biofilms decreased under the experimental conditions in the absence of exposure to external inputs of antibiotics and AMR.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Farmacorresistencia Microbiana / Biopelículas / Escherichia coli / Genes Bacterianos Idioma: En Revista: Sci Total Environ Año: 2023 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Farmacorresistencia Microbiana / Biopelículas / Escherichia coli / Genes Bacterianos Idioma: En Revista: Sci Total Environ Año: 2023 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Países Bajos