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Occurrence of Rickettsia spp. and Coxiella burnetii in ixodid ticks in Kassena-Nankana, Ghana.
Addo, Seth Offei; Bentil, Ronald Essah; Baako, Bernice Olivia Ama; Yartey, Kevin Nii; Behene, Eric; Asiamah, Benjamin; Nyarko, Abena Akyeamaa; Asoala, Victor; Sallam, Mohamed; Mate, Suzanne; Dunford, James C; Larbi, John Asiedu; Baidoo, Philip Kweku; Wilson, Michael David; Diclaro Ii, Joseph W; Dadzie, Samuel K.
Afiliación
  • Addo SO; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana. sethaddo40@gmail.com.
  • Bentil RE; Department of Theoretical and Applied Biology, College of Science, KNUST, Kumasi, Ghana. sethaddo40@gmail.com.
  • Baako BOA; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
  • Yartey KN; Navrongo Health Research Centre, Upper East Region, Navrongo, Ghana.
  • Behene E; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
  • Asiamah B; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
  • Nyarko AA; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
  • Asoala V; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
  • Sallam M; Navrongo Health Research Centre, Upper East Region, Navrongo, Ghana.
  • Mate S; Public Health and Biostatistics Department, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
  • Dunford JC; U.S. Army Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Larbi JA; Navy Entomology Center of Excellence, Jacksonville, FL, USA.
  • Baidoo PK; Uniformed Services University of Health Sciences, Bethesda, MD, USA.
  • Wilson MD; Department of Theoretical and Applied Biology, College of Science, KNUST, Kumasi, Ghana.
  • Diclaro Ii JW; Department of Theoretical and Applied Biology, College of Science, KNUST, Kumasi, Ghana.
  • Dadzie SK; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Exp Appl Acarol ; 90(1-2): 137-153, 2023 Jun.
Article en En | MEDLINE | ID: mdl-37322233
ABSTRACT
Ticks are arthropods of veterinary and medical importance which spread zoonotic pathogens that link animal and human health. In this study, ticks were collected from 448 livestock between February and December 2020 in the Kassena-Nankana Districts of Ghana and screened for the presence of zoonotic pathogens DNA using PCR and sequencing approaches. In total, 1550 ticks were collected and morphologically identified. Three tick genera were identified with Amblyomma variegatum (63%) as the predominant tick species collected. DNA was extracted from 491 tick pools and screened for the presence of DNA of Rickettsia spp. based on the 115 bp fragment of the 17 kDa surface protein and 639 bp of the Outer membrane protein A (ompA) gene and the 295 bp fragment of the transposase gene of Coxiella burnetii IS1111a element. From the 491 pools screened, the DNA of Rickettsia spp. and C. burnetii was detected in 56.8 and 3.7%, respectively. Coinfections were identified in 2.4% of the tick pools. Characterization of the Rickettsia spp. in this study based on the ompA gene showed that the DNA of Rickettsia africae and Rickettsia aeschlimannii accounted for 39.7 and 14.7%, respectively, and were 100% similar to sequences in GenBank. Most R. africae and C. burnetii infections occurred in ticks collected in the wet season, whereas R. aeschlimannii occurred mostly in the dry season. These pathogens are potential public health threats, thus there is a need to implement control measures to reduce the risk of infections in vulnerable populations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rickettsia / Garrapatas / Coxiella burnetii / Ixodidae Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: Exp Appl Acarol Asunto de la revista: BIOLOGIA / PARASITOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Ghana

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rickettsia / Garrapatas / Coxiella burnetii / Ixodidae Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: Exp Appl Acarol Asunto de la revista: BIOLOGIA / PARASITOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Ghana