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Unravelling the role of lipid composition on liposome-protein interactions.
Nele, Valeria; D'Aria, Federica; Campani, Virginia; Silvestri, Teresa; Biondi, Marco; Giancola, Concetta; De Rosa, Giuseppe.
Afiliación
  • Nele V; BioNanoMed Drug Delivery Group, Department of Pharmacy, University of Naples Federico II, Naples, Italy.
  • D'Aria F; Biophysics Laboratory, Department of Pharmacy, University of Naples Federico II, Naples, Italy.
  • Campani V; BioNanoMed Drug Delivery Group, Department of Pharmacy, University of Naples Federico II, Naples, Italy.
  • Silvestri T; BioNanoMed Drug Delivery Group, Department of Pharmacy, University of Naples Federico II, Naples, Italy.
  • Biondi M; BioNanoMed Drug Delivery Group, Department of Pharmacy, University of Naples Federico II, Naples, Italy.
  • Giancola C; Biophysics Laboratory, Department of Pharmacy, University of Naples Federico II, Naples, Italy.
  • De Rosa G; BioNanoMed Drug Delivery Group, Department of Pharmacy, University of Naples Federico II, Naples, Italy.
J Liposome Res ; 34(1): 88-96, 2024 Mar.
Article en En | MEDLINE | ID: mdl-37337884
ABSTRACT
Upon in vivo administration of nanoparticles, a protein corona forms on their surface and affects their half-life in circulation, biodistribution properties, and stability; in turn, the composition of the protein corona depends on the physico-chemical properties of the nanoparticles. We have previously observed lipid composition-dependent in vitro and in vivo microRNA delivery from lipid nanoparticles. Here, we carried out an extensive physico-chemical characterisation to understand the role of the lipid composition on the in vivo fate of lipid-based nanoparticles. We used a combination of differential scanning calorimetry (DSC), membrane deformability measurements, isothermal titration calorimetry (ITC), and dynamic light scattering (DLS) to probe the interactions between the nanoparticle surface and bovine serum albumin (BSA) as a model protein. The lipid composition influenced membrane deformability, improved lipid intermixing, and affected the formation of lipid domains while BSA binding to the liposome surface was affected by the PEGylated lipid content and the presence of cholesterol. These findings highlight the importance of the lipid composition on the protein-liposome interaction and provide important insights for the design of lipid-based nanoparticles for drug delivery applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas / Corona de Proteínas Tipo de estudio: Prognostic_studies Idioma: En Revista: J Liposome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Italia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas / Corona de Proteínas Tipo de estudio: Prognostic_studies Idioma: En Revista: J Liposome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Italia