Sludge granulation in PN/A enhances nitrogen removal from mainstream anaerobically pretreated wastewater.

Treating anaerobically pretreated wastewater using partial nitritation/anammox (PN/A) process faces severe challenges because of the complex syntrophic and competitive relationship among various bacteria. Results of this study suggested a continuous low dissolved oxygen (DO) concentration failed to sustain NH4+ removal (<80 %), whereas moderate DO concentrations with high aerobic periods suppressed anammox reaction. Through implementing a moderate DO concentration with low aerobic periods (MDO-LA), NH4+ and total nitrogen removal efficiency reached 91.5 ± 5.5 % and 71.3 ± 2.8 % respectively. The specific activities of ammonium-oxidizing bacteria (AOB) and anaerobic ammonium-oxidizing bacteria (AnAOB) reached 0.942 ± 0.030 and 0.277 ± 0.010 g nitrogen per gram mixed liquor volatile suspended solids, respectively, mainly because MDO-LA favored Thiothrix (filamentous bacteria) wash-out and promoted Nitrosomonas growth. Moreover, sludge granules covered by a thin exterior rim with abundant AOB were formed, favoring Ca. Brocadia growth (5.4 % to 13.2 %) and mass transfer between AOB and AnAOB, which consequently increased the expression of genes coding hydroxylamine oxidase and hydrazine synthase. Overall, achievements in this study provide a promising operating strategy for PN/A treating anaerobically pretreated wastewater.