Your browser doesn't support javascript.
loading
A spatially resolved single-cell genomic atlas of the adult human breast.
Kumar, Tapsi; Nee, Kevin; Wei, Runmin; He, Siyuan; Nguyen, Quy H; Bai, Shanshan; Blake, Kerrigan; Pein, Maren; Gong, Yanwen; Sei, Emi; Hu, Min; Casasent, Anna K; Thennavan, Aatish; Li, Jianzhuo; Tran, Tuan; Chen, Ken; Nilges, Benedikt; Kashikar, Nachiket; Braubach, Oliver; Ben Cheikh, Bassem; Nikulina, Nadya; Chen, Hui; Teshome, Mediget; Menegaz, Brian; Javaid, Huma; Nagi, Chandandeep; Montalvan, Jessica; Lev, Tatyana; Mallya, Sharmila; Tifrea, Delia F; Edwards, Robert; Lin, Erin; Parajuli, Ritesh; Hanson, Summer; Winocour, Sebastian; Thompson, Alastair; Lim, Bora; Lawson, Devon A; Kessenbrock, Kai; Navin, Nicholas.
Afiliación
  • Kumar T; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Nee K; Graduate School of Biomedical Sciences, University of Texas MD Anderson Cancer Center, Houston, TX, USA.
  • Wei R; Department of Biological Chemistry, University of California, Irvine, Irvine, CA, USA.
  • He S; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Nguyen QH; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Bai S; Graduate School of Biomedical Sciences, University of Texas MD Anderson Cancer Center, Houston, TX, USA.
  • Blake K; Department of Biological Chemistry, University of California, Irvine, Irvine, CA, USA.
  • Pein M; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Gong Y; Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, USA.
  • Sei E; Math, Computational & Systems Biology, University of California, Irvine, Irvine, CA, USA.
  • Hu M; Department of Biological Chemistry, University of California, Irvine, Irvine, CA, USA.
  • Casasent AK; Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, USA.
  • Thennavan A; Department of Biological Chemistry, University of California, Irvine, Irvine, CA, USA.
  • Li J; Math, Computational & Systems Biology, University of California, Irvine, Irvine, CA, USA.
  • Tran T; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Chen K; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Nilges B; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Kashikar N; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Braubach O; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Ben Cheikh B; Department of Systems Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Nikulina N; Department of Bioinformatics and Computational Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Chen H; Resolve Biosciences, Monheim am Rhein, Germany.
  • Teshome M; Resolve Biosciences, Monheim am Rhein, Germany.
  • Menegaz B; Akoya Biosciences, Menlo Park, CA, USA.
  • Javaid H; Akoya Biosciences, Menlo Park, CA, USA.
  • Nagi C; Akoya Biosciences, Menlo Park, CA, USA.
  • Montalvan J; Department of Pathology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Lev T; Department of Breast Surgical Oncology, UT MD Anderson Cancer Center, Houston, TX, USA.
  • Mallya S; Department of Pathology and Immunology, Baylor Medical College, Houston, TX, USA.
  • Tifrea DF; Department of Pathology and Immunology, Baylor Medical College, Houston, TX, USA.
  • Edwards R; Department of Pathology and Immunology, Baylor Medical College, Houston, TX, USA.
  • Lin E; Department of Pathology and Immunology, Baylor Medical College, Houston, TX, USA.
  • Parajuli R; Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, USA.
  • Hanson S; Math, Computational & Systems Biology, University of California, Irvine, Irvine, CA, USA.
  • Winocour S; Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, USA.
  • Thompson A; Chao Comprehensive Cancer Center, University of California, Irvine, Irvine, CA, USA.
  • Lim B; Chao Comprehensive Cancer Center, University of California, Irvine, Irvine, CA, USA.
  • Lawson DA; Chao Comprehensive Cancer Center, University of California, Irvine, Irvine, CA, USA.
  • Kessenbrock K; Chao Comprehensive Cancer Center, University of California, Irvine, Irvine, CA, USA.
  • Navin N; Department of Surgery, University of Chicago Medicine, Chicago, IL, USA.
Nature ; 620(7972): 181-191, 2023 Aug.
Article en En | MEDLINE | ID: mdl-37380767
ABSTRACT
The adult human breast is comprised of an intricate network of epithelial ducts and lobules that are embedded in connective and adipose tissue1-3. Although most previous studies have focused on the breast epithelial system4-6, many of the non-epithelial cell types remain understudied. Here we constructed the comprehensive Human Breast Cell Atlas (HBCA) at single-cell and spatial resolution. Our single-cell transcriptomics study profiled 714,331 cells from 126 women, and 117,346 nuclei from 20 women, identifying 12 major cell types and 58 biological cell states. These data reveal abundant perivascular, endothelial and immune cell populations, and highly diverse luminal epithelial cell states. Spatial mapping using four different technologies revealed an unexpectedly rich ecosystem of tissue-resident immune cells, as well as distinct molecular differences between ductal and lobular regions. Collectively, these data provide a reference of the adult normal breast tissue for studying mammary biology and diseases such as breast cancer.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mama / Perfilación de la Expresión Génica / Análisis de la Célula Individual Límite: Adult / Female / Humans Idioma: En Revista: Nature Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mama / Perfilación de la Expresión Génica / Análisis de la Célula Individual Límite: Adult / Female / Humans Idioma: En Revista: Nature Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos