Your browser doesn't support javascript.
loading
Exogenous abscisic acid represses rice flowering via SAPK8-ABF1-Ehd1/Ehd2 pathway.
Tang, Liqun; Li, Guanghao; Wang, Huimei; Zhao, Juan; Li, Zhiyong; Liu, Xixi; Shu, Yazhou; Liu, Wanning; Wang, Shuang; Huang, Jie; Ying, Jiezheng; Tong, Xiaohong; Yuan, Wenya; Wei, Xiangjin; Tang, Shaoqing; Wang, Yifeng; Bu, Qingyun; Zhang, Jian.
Afiliación
  • Tang L; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Li G; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Wang H; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Zhao J; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Li Z; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Liu X; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Shu Y; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Liu W; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Wang S; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Huang J; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Ying J; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Tong X; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Yuan W; Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 430062, China.
  • Wei X; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Tang S; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Wang Y; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China. Electronic address: wangyifeng@caas.cn.
  • Bu Q; Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, the Chinese Academy of Sciences, Harbin 150081, China; The Innovative Academy of Seed Design, the Chinese Academy of Sciences, Beijing 100101, China. Electronic address: buqingyun@iga.ac.cn.
  • Zhang J; State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China. Electronic address: zhangjian@caas.cn.
J Adv Res ; 2023 Jul 01.
Article en En | MEDLINE | ID: mdl-37399924
ABSTRACT

INTRODUCTION:

Rice flowering is a major agronomic trait, determining yield and ecological adaptability in particular regions. ABA plays an essential role in rice flowering, but the underlying molecular mechanism remains largely elusive.

OBJECTIVES:

In this study, we demonstrated a "SAPK8-ABF1-Ehd1/Ehd2" pathway, through which exogenous ABA represses rice flowering in a photoperiod-independent manner.

METHODS:

We generated abf1 and sapk8 mutants using the CRISPR-Cas9 method. Using yeast two-hybrid, Pull down, BiFC and kinase assays, SAPK8 interacted and phosphorylated ABF1. ABF1 directly bound to the promoters of Ehd1 and Ehd2 using ChIP-qPCR, EMSA, and LUC transient transcriptional activity assay, and suppressed the transcription of these genes.

RESULTS:

Under both long day and short day conditions, simultaneous knock-out of ABF1 and its homolog bZIP40 accelerated flowering, while SAPK8 and ABF1 over-expression lines exhibited delayed flowering and hypersensitivity to ABA-mediated flowering repression. After perceiving the ABA signal, SAPK8 physically binds to and phosphorylates ABF1 to enhance its binding to the promoters of master positive flowering regulators Ehd1 and Ehd2. Upon interacting with FIE2, ABF1 recruited PRC2 complex to deposit H3K27me3 suppressive histone modification on Ehd1 and Ehd2 to suppress these genes transcription, thereby leading to later flowering.

CONCLUSION:

Our work highlighted the biological functions of SAPK8 and ABF1 in ABA signaling, flowering control and the involvement of a PRC2-mediated epigenetic repression mechanism in the transcription regulation governed by ABF1 on ABA-mediated rice flowering repression.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Adv Res Año: 2023 Tipo del documento: Article País de afiliación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Adv Res Año: 2023 Tipo del documento: Article País de afiliación: China