Generation of Human 293-F Suspension NGFR Knockout Cells Using CRISPR/Cas9 Coupled to Fluorescent Protein Expression.

Schatz, Stefanie; van Dijk, Femke Harmina; Dubiel, Aleksandra Elzbieta; Cantz, Tobias; Eggenschwiler, Reto; Stitz, Jörn

Schatz, Stefanie; van Dijk, Femke Harmina; Dubiel, Aleksandra Elzbieta; Cantz, Tobias; Eggenschwiler, Reto; Stitz, Jörn.

Afiliación

Schatz S; Research Group Medical Biotechnology & Bioengineering, Faculty of Applied Natural Sciences, TH Köln - University of Applied Sciences, Campus Leverkusen, Leverkusen, Germany.
van Dijk FH; Institute of Technical Chemistry, Leibniz University Hannover, Hannover, Germany.
Dubiel AE; Research Group Medical Biotechnology & Bioengineering, Faculty of Applied Natural Sciences, TH Köln - University of Applied Sciences, Campus Leverkusen, Leverkusen, Germany.
Cantz T; Research Group Medical Biotechnology & Bioengineering, Faculty of Applied Natural Sciences, TH Köln - University of Applied Sciences, Campus Leverkusen, Leverkusen, Germany.
Eggenschwiler R; Research Group Translational Hepatology and Stem Cell Biology, Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany.
Stitz J; Research Group Translational Hepatology and Stem Cell Biology, Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany.

Methods Mol Biol ; 2681: 361-371, 2023.

Article en En | MEDLINE | ID: mdl-37405658

Asunto(s)

Sistemas CRISPR-Cas; Edición Génica; Humanos; Sistemas CRISPR-Cas/genética; Edición Génica/métodos; Receptor de Factor de Crecimiento Nervioso/genética; Células HEK293; Vectores Genéticos/genética; Receptores de Factor de Crecimiento Nervioso/genética; Proteínas del Tejido Nervioso/genética

Palabras clave

293-F; CRISPR/Cas9; Gene editing; Gene therapy; Human suspension cells; Large-scale production; Lentiviral vectors; NGFR; Packaging cell; Retroviral vectors

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistemas CRISPR-Cas / Edición Génica Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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