Your browser doesn't support javascript.
loading
Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology.
Alowaysi, Maryam; Al-Shehri, Mohammad; Baadhaim, Moayad; AlZahrani, Hajar; Aboalola, Doaa; Daghestani, Mustafa; Hashem, Heba; Aljahdali, Rawan; Salem, Rayan; Alharbi, Adel; Muharraq, Mohammed; Alghamdi, Khaled; Alsobiy, Fawaz; Zia, Asima; Lehmann, Robert; Tegner, Jesper; Alsayegh, Khaled.
Afiliación
  • Alowaysi M; King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
  • Al-Shehri M; King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
  • Baadhaim M; King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
  • AlZahrani H; King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
  • Aboalola D; King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
  • Daghestani M; King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia; Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia.
  • Hashem H; Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia.
  • Aljahdali R; Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia.
  • Salem R; Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia.
  • Alharbi A; Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia.
  • Muharraq M; Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia.
  • Alghamdi K; Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia.
  • Alsobiy F; Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia.
  • Zia A; Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
  • Lehmann R; Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
  • Tegner J; Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
  • Alsayegh K; King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia. Electronic address: alsayeghkh@mngha.med.sa.
Stem Cell Res ; 71: 103158, 2023 09.
Article en En | MEDLINE | ID: mdl-37406498
ABSTRACT
Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB's role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB-/-) via CRISPR/Cas9 to study MB function in human biology and diseases.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Embrionarias Humanas / Mioglobina Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2023 Tipo del documento: Article País de afiliación: Arabia Saudita
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Embrionarias Humanas / Mioglobina Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2023 Tipo del documento: Article País de afiliación: Arabia Saudita