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COVID-19: S-Peptide RBD 484-508 Induces IFN-γ T-Cell Response in Naïve-to-Infection and Unvaccinated Subjects with Close Contact with SARS-CoV-2-Positive Patients.
Murdocca, Michela; Citro, Gennaro; Centanini, Eleonora; Giannini, Rosalinda; Latini, Andrea; Centofanti, Federica; Piano Mortari, Eva; Cocciadiferro, Dario; Novelli, Antonio; Bernardini, Sergio; Novelli, Giuseppe; Sangiuolo, Federica.
Afiliación
  • Murdocca M; Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
  • Citro G; Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
  • Centanini E; Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
  • Giannini R; Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
  • Latini A; Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
  • Centofanti F; Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
  • Piano Mortari E; B Cell Unit, Immunology Research Area, Bambino Gesù Children's Hospital, IRCCS, 00165 Rome, Italy.
  • Cocciadiferro D; Medical Genetics, Bambino Gesù Children's Hospital, IRCCS, 00165 Rome, Italy.
  • Novelli A; Medical Genetics, Bambino Gesù Children's Hospital, IRCCS, 00165 Rome, Italy.
  • Bernardini S; Department of Experimental Medicine, University of Tor Vergata, 00133 Rome, Italy.
  • Novelli G; Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
  • Sangiuolo F; IRCCS Neuromed, 86077 Pozzilli, Italy.
Viruses ; 15(7)2023 06 22.
Article en En | MEDLINE | ID: mdl-37515105
Despite the availability on the market of different anti-SARS-CoV-2 vaccines, there are still unanswered questions on whether they can stimulate long-lasting protection. A deep understanding of adaptive immune response to SARS-CoV-2 is important for optimizing both vaccine development and pandemic control measures. Among cytokines secreted by lymphocytes in response to viral infection, IFN-γ plays a pivotal role both in innate and adaptive immunity. In this study, we report on 28 naïve-to-SARS-Cov-2-infection and unvaccinated subjects, having reported a close and prolonged contact with COVID-19-positive patients. Samples were tested for defective genetic variants in interferon pathway genes by whole exome sequencing and anti-IFN autoantibodies production was investigated. Subject T-cells were cultured and infected with pseudotype particles bearing the S proteins and in parallel stimulated with two S-peptides designed on the RBD region of the spike protein. Our results showed that one of these peptides, RBD 484-508, induces a significant increase in IFN-γ gene expression and protein production in T-cells, comparable to those obtained in cells infected by SARS-CoV-2 pseudovirus. This work deepens our understanding of immune response and highlights the selected peptide as a reasonable approach to induce broad, potent, and variant concern-independent T-cell responses.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: COVID-19 Límite: Humans Idioma: En Revista: Viruses Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: COVID-19 Límite: Humans Idioma: En Revista: Viruses Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza