Protocol for constructing a hierarchical host-guest supramolecular self-assembly system in water.

Here, we present a protocol for the construction of a hierarchical host-guest supramolecular self-assembly system in water. We describe steps for determining the binding levels and capturing the morphologies of hierarchical self-assembly. We detail procedures for using UV-vis spectra, nuclear magnetic resonance spectra, scanning electron microscopy, and transmission electron microscopy for the assembly. This protocol is useful for analyzing the detailed chemical structure and morphological variation of hierarchical host-guest supramolecular self-assembly systems. For complete details on the use and execution of this protocol, please refer to Chen et al. (2022).1.