CRISPR generation of CSF1R-G795A human microglia for robust microglia replacement in a chimeric mouse model.
STAR Protoc
; 4(3): 102490, 2023 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-37516973
ABSTRACT
Chimeric mouse models have recently been developed to study human microglia in vivo. However, widespread engraftment of donor microglia within the adult brain has been challenging. Here, we present a protocol to introduce the G795A point mutation using CRISPR-Cas9 into the CSF1R locus of human pluripotent stem cells. We also describe an optimized microglial differentiation technique for transplantation into newborn or adult recipients. We then detail pharmacological paradigms to achieve widespread and near-complete engraftment of human microglia. For complete details on the use and execution of this protocol, please refer to Chadarevian et al. (2023).1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microglía
/
Células Madre Pluripotentes
Límite:
Adult
/
Animals
/
Humans
/
Newborn
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article