Rapid expression of novel proteins in goldfish retina following optic nerve crush.
Biochem Biophys Res Commun
; 138(3): 1177-83, 1986 Aug 14.
Article
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| MEDLINE
| ID: mdl-3753493
ABSTRACT
Polyadenylated messenger RNA was isolated from goldfish retinas at various times following unilateral crush of the optic nerve. RNA was translated in a cell-free system and product proteins analyzed by two-dimensional gel electrophoresis and autofluorography. Poly(A)+ mRNA-directed protein synthesis revealed an 8-fold increase in the labeling of polypeptides of about 30 kd Mr and a pI of 5.5 in retinas 2 d following optic nerve crush, compared with control retina mRNA translation products. In vitro labeling of retinal proteins revealed the enhanced synthesis of comparable 30 kd proteins in 2 d post-crush retinas. Evidence presented suggests that this 30 kd protein cluster may correspond to fish 30 kd stress or heat-shock proteins (hsp-30).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nervio Óptico
/
Retina
/
Compresión Nerviosa
/
Regeneración Nerviosa
/
Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1986
Tipo del documento:
Article