Crosstalk between cholesterol and PIP2 in the regulation of Kv7.2/Kv7.3 channels.
Biol Chem
; 405(3): 161-165, 2024 Mar 25.
Article
en En
| MEDLINE
| ID: mdl-37552610
ABSTRACT
The activity of neuronal Kv7.2/Kv7.3 channels is critically dependent on PIP2 and finely modulated by cholesterol. Here, we report the crosstalk between cholesterol and PIP2 in the regulation of Kv7.2/Kv7.3 channels. Our results show that currents passing through Kv7.2/Kv7.3 channels in cholesterol-depleted cells, by acute application of methyl-ß-cyclodextrin (MßCD), were less sensitive to PIP2 dephosphorylation strategies than those of control cells, suggesting that cholesterol depletion enhances the Kv7.2/Kv7.3-PIP2 interaction. In contrast, the sensitivity of Kv7.2/Kv7.3 channels to acute membrane cholesterol depletion by MßCD was not altered in mutant channels with different apparent affinities for PIP2.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colesterol
Idioma:
En
Revista:
Biol Chem
Asunto de la revista:
BIOQUIMICA
Año:
2024
Tipo del documento:
Article