Ultrasensitive levofloxacin electrochemical biosensor based on semiconducting covalent organic framework/poly-L-cysteine/triangular Ag nanoplates modified glassy carbon electrode.
Mikrochim Acta
; 190(9): 346, 2023 08 09.
Article
en En
| MEDLINE
| ID: mdl-37555996
ABSTRACT
A novel electrochemical biosensor with excellent performance was fabricated for levofloxacin (LEV) detection, which adopted triangular Ag nanoplates (Tri-AgNP) confined in a poly-L-cysteine (poly-L-Cys) film and a semiconducting covalent organic framework (COF) as the electrochemical sensing material. The developed electrochemical sensor revealed excellent analytical properties because of its good electrical conductivity, fast electron transfer, and abundant bioactive site. Based on this, a linear relationship between the LEV concentration and the peak current response at 0.92 V was obtained under the optimal experimental conditions by differential pulse voltammetry (DPV), with a wide linear range of 0.05 to 600 µM and a low limit of detection (LOD) of 0.0061 µM. The prepared sensor also realized sensitive and accurate determination of LEV in human serum and urine samples by standard addition method, with satisfactory recoveries (97.1 to 104%) and a low relative standard deviation of less than 4.6%. These results indicated that the novel ternary system has a promising application in the development of electrochemical signal probe and electrochemical biosensing platform.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Estructuras Metalorgánicas
Límite:
Humans
Idioma:
En
Revista:
Mikrochim Acta
Año:
2023
Tipo del documento:
Article