Your browser doesn't support javascript.
loading
CREB-binding protein and HIF-1α/ß-catenin to upregulate miR-322 and alleviate myocardial ischemia-reperfusion injury.
Dong, Wei; Weng, Jun-Fei; Zhu, Jian-Bing; Zheng, Yao-Fu; Liu, Lei-Lei; Dong, Chen; Ruan, Yang; Fang, Xu; Chen, Jin; Liu, Wen-Yu; Peng, Xiao-Ping; Chen, Xuan-Ying.
Afiliación
  • Dong W; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Weng JF; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Zhu JB; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Zheng YF; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Liu LL; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Dong C; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Ruan Y; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Fang X; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Chen J; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Liu WY; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Peng XP; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
  • Chen XY; Department of Pharmacy, The First Affiliated Hospital of Nanchang University, Nanchang, China.
FASEB J ; 37(9): e22996, 2023 09.
Article en En | MEDLINE | ID: mdl-37566526
Myocardial ischemia/reperfusion injury (MIRI) is a prevalent condition associated with numerous critical clinical conditions. miR-322 has been implicated in MIRI through poorly understood mechanisms. Our preliminary analysis indicated potential interaction of CREB-binding protein (CBP), a transcriptional coactivator and acetyltransferase, with HIF-1α/ß-catenin, which might regulate miR-322 expression. We, therefore, hypothesized that CBP/HIF-1α/ß-catenin/miR-322 axis might play a role in MIRI. Rat cardiomyocytes subjected to oxygen-glucose deprivation /reperfusion (OGD/R) and Langendorff perfused heart model were used to model MIRI in vitro and in vivo, respectively. We used various techniques such as CCK-8 assay, transferase dUTP nick end labeling staining, western blotting, RT-qPCR, chromatin immunoprecipitation (ChIP), dual-luciferase assay, co-immunoprecipitation (Co-IP), hematoxylin and eosin staining, and TTC staining to assess cell viability, apoptosis, and the levels of CBP, HIF-1α, ß-catenin, miR-322, and acetylation. Our results indicate that OGD/R in cardiomyocytes decreased CBP/HIF-1α/ß-catenin/miR-322 expression, increased cell apoptosis and cytokines, and reduced cell viability. However, overexpression of CBP or miR-322 suppressed OGD/R-induced cell injury, while knockdown of HIF-1α/ß-catenin further exacerbated the damage. HIF-1α/ß-catenin bound to miR-322 promoter to promote its expression, while CBP acetylated HIF-1α/ß-catenin for stabilization. Overexpression of CBP attenuated MIRI in rats by acetylating HIF-1α/ß-catenin to stabilize their expression, resulting in stronger binding of HIF-1α/ß-catenin with the miR-322 promoter and subsequent increased miR-322 levels. Therefore, activating CBP/HIF-1α/ß-catenin/miR-322 signaling may be a potential approach to treat MIRI.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión Miocárdica / MicroARNs Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión Miocárdica / MicroARNs Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos