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Acid-resistant enzymes: the acquisition strategies and applications.
Zhang, Zhenzhen; Zhao, Zitong; Huang, Kunlun; Liang, Zhihong.
Afiliación
  • Zhang Z; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
  • Zhao Z; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
  • Huang K; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
  • Liang Z; The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing, China.
Appl Microbiol Biotechnol ; 107(20): 6163-6178, 2023 Oct.
Article en En | MEDLINE | ID: mdl-37615723
ABSTRACT
Enzymes have promising applications in chemicals, food, pharmaceuticals, and other variety products because of their high efficiency, specificity, and environmentally friendly properties. However, due to the complexity of raw materials, pH, temperature, solvents, etc., the application range of enzymes is greatly limited in the industry. Protein engineering and enzyme immobilization are classical strategies to overcome the limitations of industrial applications. Although the pH tendency of enzymes has been extensively researched, the mechanism underlying enzyme acid resistance is unclear, and a less practical strategy for altering the pH propensity of enzymes has been suggested. This review proposes that the optimum pH of enzyme is determined by the pKa values of active center ionizable amino acid residues. Three levels of acquiring acid-resistant enzymes are summarized mining from extreme environments and enzyme databases, modification with protein engineering and enzyme microenvironment engineering, and de novo synthesis. The industrial applications of acid-resistant enzymes in chemicals, food, and pharmaceuticals are also summarized. KEY POINTS • The mechanism of enzyme acid resistance is fundamentally determined. • The three aspects of the method for acquiring acid-resistant enzymes are summarized. • Computer-aided strategies and artificial intelligence are used to obtain acid-resistant enzymes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inteligencia Artificial / Enzimas Inmovilizadas Idioma: En Revista: Appl Microbiol Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inteligencia Artificial / Enzimas Inmovilizadas Idioma: En Revista: Appl Microbiol Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: China