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A 3D-Printed Assemblable Bespoke Scaffold as a Versatile Microcryogel Carrier for Site-Specific Regenerative Medicine.
Lee, Seunghun S; Kleger, Nicole; Kuhn, Gisela A; Greutert, Helen; Du, Xiaoyu; Smit, Thijs; Studart, André R; Ferguson, Stephen J.
Afiliación
  • Lee SS; Institute for Biomechanics, Department of Health Sciences and Technology, ETH Zurich, Zurich, 8092, Switzerland.
  • Kleger N; Complex Materials, Department of Materials, ETH Zurich, Zurich, 8093, Switzerland.
  • Kuhn GA; Institute for Biomechanics, Department of Health Sciences and Technology, ETH Zurich, Zurich, 8092, Switzerland.
  • Greutert H; Institute for Biomechanics, Department of Health Sciences and Technology, ETH Zurich, Zurich, 8092, Switzerland.
  • Du X; Institute for Biomechanics, Department of Health Sciences and Technology, ETH Zurich, Zurich, 8092, Switzerland.
  • Smit T; Institute for Biomechanics, Department of Health Sciences and Technology, ETH Zurich, Zurich, 8092, Switzerland.
  • Studart AR; Complex Materials, Department of Materials, ETH Zurich, Zurich, 8093, Switzerland.
  • Ferguson SJ; Institute for Biomechanics, Department of Health Sciences and Technology, ETH Zurich, Zurich, 8092, Switzerland.
Adv Mater ; 35(44): e2302008, 2023 Nov.
Article en En | MEDLINE | ID: mdl-37632210
Advances in additive manufacturing have led to diverse patient-specific implant designs utilizing computed tomography, but this requires intensive work and financial implications. Here, Digital Light Processing is used to fabricate a hive-structured assemblable bespoke scaffold (HIVE). HIVE can be manually assembled in any shape/size with ease, so a surgeon can create a scaffold that will best fit a defect before implantation. Simultaneously, it can have site-specific treatments by working as a carrier filled with microcryogels (MC) incorporating different biological factors in different pockets of HIVE. After characterization, possible site-specific applications are investigated by utilizing HIVE as a versatile carrier with incorporated treatments such as growth factors (GF), bioceramic, or cells. HIVE as a GF-carrier shows a controlled release of bone morphogenetic protein/vascular endothelial growth factor (BMP/VEGF) and induced osteogenesis/angiogenesis from human mesenchymal stem cells (hMSC)/human umbilical vein endothelial cells (HUVECs). Furthermore, as a bioceramic-carrier, HIVE demonstrates enhanced mineralization and osteogenesis, and as a HUVEC carrier, it upregulates both osteogenic and angiogenic gene expression of hMSCs. HIVE with different combinations of MCs yields a distinct local effect and successful cell migration is confirmed within assembled HIVEs. Finally, an in vivo rat subcutaneous implantation demonstrates site-specific osteogenesis and angiogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Medicina Regenerativa / Andamios del Tejido Límite: Animals / Humans Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Medicina Regenerativa / Andamios del Tejido Límite: Animals / Humans Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Alemania