Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages.

Busch, Clara Jana-Lui; Subramanian, Sethuraman; Linares, Javier; Favret, Jérémy; Yuda, Ridzky Anis Advent; Sieweke, Michael H

Busch, Clara Jana-Lui; Subramanian, Sethuraman; Linares, Javier; Favret, Jérémy; Yuda, Ridzky Anis Advent; Sieweke, Michael H.

Afiliación

Busch CJ; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
Subramanian S; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
Linares J; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
Favret J; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
Yuda RAA; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
Sieweke MH; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany. michael.sieweke@tu-dresden.de.

Methods Mol Biol ; 2713: 231-251, 2024.

Article en En | MEDLINE | ID: mdl-37639127

ABSTRACT

ABSTRACT

Alveolar macrophages (AM) are resident macrophages of the lung and play important roles in the maintenance of tissue homeostasis as well as host defense. Here, we describe how they can be harvested from murine lungs, expanded in vitro, and transduced with lentiviral vectors.

Asunto(s)

Macrófagos Alveolares; Macrófagos; Animales; Ratones; Tórax

Palabras clave

Alveolar macrophage; Bronchoalveolar lavage; Conditioned medium; GM-CSF; Genetic engineering; Genetic manipulation; Lentiviral transduction; Lungs; Primary cell culture; Self-renewal; Tissue-resident macrophage

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Macrófagos Alveolares / Macrófagos Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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