17ß-Trenbolone activates androgen receptor, upregulates transforming growth factor beta/bone morphogenetic protein and Wnt signaling pathways, and induces masculinization of caudal and anal fins in female guppies (Poecilia reticulata).
Aquat Toxicol
; 263: 106677, 2023 Oct.
Article
en En
| MEDLINE
| ID: mdl-37677862
ABSTRACT
Sexually mature female guppies (Poecilia reticulata) were exposed to environmentally relevant concentrations (20, 200, and 2000 ng/L) of 17ß-trenbolone for four weeks. As evidenced by the increased caudal fin index and anal fins developing into gonopodium-like structures, exposed females displayed masculinized secondary sexual characteristics. Differential gene expression and subsequent pathway analysis of mRNA sequencing data revealed that the transcription of transforming growth factor beta/bone morphogenetic protein signaling pathway and Wnt signaling pathway were upregulated following 17ß-trenbolone exposure. Enzyme-linked immunosorbent assays showed that the bone morphogenetic protein 7 protein content was elevated after 17ß-trenbolone exposure. Finally, real-time PCR revealed that 17ß-trenbolone treatment significantly increased androgen receptor mRNA levels, and molecular docking showed potent interaction between 17ß-trenbolone and guppy androgen receptor. Furthermore, 17ß-trenbolone-induced masculinization of caudal and anal fins in female guppies, concomitant to the upregulated expression of differentially expressed genes involved in the above-mentioned two signaling pathways, was significantly inhibited by flutamide (androgen receptor antagonist). These findings demonstrated that 17ß-trenbolone masculinized fins of female guppies by activating the androgen receptor. This study revealed that 17ß-trenbolone could upregulate signaling pathways related to fin growth and differentiation, and eventually cause caudal and anal fin masculinization in female guppies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Contaminantes Químicos del Agua
/
Poecilia
Límite:
Animals
Idioma:
En
Revista:
Aquat Toxicol
Asunto de la revista:
BIOLOGIA
/
TOXICOLOGIA
Año:
2023
Tipo del documento:
Article
País de afiliación:
China