Protocol for in vivo and in vitro activation of NLRP3 inflammasome in mice using monosodium urate.
STAR Protoc
; 4(3): 102554, 2023 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-37682717
ABSTRACT
Although intraperitoneal injection of monosodium urate (MSU) is an effective model for studying peritonitis, its establishment remains challenging. Here, we present a protocol for using MSU to activate the NLRP3 inflammasome in bone-marrow-derived macrophages (BMDMs) and to induce peritonitis in mice. We describe steps for isolating and culturing BMDMs, preparing MSU crystals, and activating the NLRP3 inflammasome using western blot and ELISA. We then detail procedures for inducing peritonitis and testing for relevant indicators using flow cytometry and ELISA. For complete details on the use and execution of this protocol, please refer to Huang et al. (2023).1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Peritonitis
/
Inflamasomas
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article
País de afiliación:
China