Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans.
STAR Protoc
; 4(4): 102576, 2023 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-37733596
ABSTRACT
The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Centro Germinal
/
Ganglios Linfáticos
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article