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A cellular resolution atlas of Broca's area.
Costantini, Irene; Morgan, Leah; Yang, Jiarui; Balbastre, Yael; Varadarajan, Divya; Pesce, Luca; Scardigli, Marina; Mazzamuto, Giacomo; Gavryusev, Vladislav; Castelli, Filippo Maria; Roffilli, Matteo; Silvestri, Ludovico; Laffey, Jessie; Raia, Sophia; Varghese, Merina; Wicinski, Bridget; Chang, Shuaibin; Chen, Ichun Anderson; Wang, Hui; Cordero, Devani; Vera, Matthew; Nolan, Jackson; Nestor, Kimberly; Mora, Jocelyn; Iglesias, Juan Eugenio; Garcia Pallares, Erendira; Evancic, Kathryn; Augustinack, Jean C; Fogarty, Morgan; Dalca, Adrian V; Frosch, Matthew P; Magnain, Caroline; Frost, Robert; van der Kouwe, Andre; Chen, Shih-Chi; Boas, David A; Pavone, Francesco Saverio; Fischl, Bruce; Hof, Patrick R.
Afiliación
  • Costantini I; European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino (FI), Italy.
  • Morgan L; Department of Biology, University of Florence, Florence, Italy.
  • Yang J; National Institute of Optics (INO), National Research Council (CNR), Sesto Fiorentino, Italy.
  • Balbastre Y; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
  • Varadarajan D; Department of Biomedical Engineering, Boston University, Boston, MA, USA.
  • Pesce L; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
  • Scardigli M; Department of Radiology, Harvard Medical School, Boston, MA, USA.
  • Mazzamuto G; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
  • Gavryusev V; Department of Radiology, Harvard Medical School, Boston, MA, USA.
  • Castelli FM; European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino (FI), Italy.
  • Roffilli M; European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino (FI), Italy.
  • Silvestri L; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino (FI), Italy.
  • Laffey J; Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
  • Raia S; European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino (FI), Italy.
  • Varghese M; National Institute of Optics (INO), National Research Council (CNR), Sesto Fiorentino, Italy.
  • Wicinski B; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino (FI), Italy.
  • Chang S; European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino (FI), Italy.
  • Chen IA; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino (FI), Italy.
  • Wang H; European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino (FI), Italy.
  • Cordero D; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino (FI), Italy.
  • Vera M; Bioretics srl, Cesena, Italy.
  • Nolan J; Bioretics srl, Cesena, Italy.
  • Nestor K; European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino (FI), Italy.
  • Mora J; National Institute of Optics (INO), National Research Council (CNR), Sesto Fiorentino, Italy.
  • Iglesias JE; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino (FI), Italy.
  • Garcia Pallares E; Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Evancic K; Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Augustinack JC; Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Fogarty M; Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Dalca AV; Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA.
  • Frosch MP; Department of Biomedical Engineering, Boston University, Boston, MA, USA.
  • Magnain C; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
  • Frost R; Department of Radiology, Harvard Medical School, Boston, MA, USA.
  • van der Kouwe A; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
  • Chen SC; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
  • Boas DA; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
  • Pavone FS; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
  • Fischl B; Department of Radiology, Harvard Medical School, Boston, MA, USA.
  • Hof PR; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA.
Sci Adv ; 9(41): eadg3844, 2023 10 13.
Article en En | MEDLINE | ID: mdl-37824623
ABSTRACT
Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)-referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custom-made inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca's area in a human postmortem specimen, within a whole-brain reference space atlas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Corteza Cerebral / Área de Broca Límite: Humans Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Italia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Corteza Cerebral / Área de Broca Límite: Humans Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Italia