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Evaluation of phenotypical and genotypical methods for the identification and typing of Stenotrophomonas maltophilia isolated from a pharmaceutical facility.
de Miranda, Rebeca Vitória da Silva Lage; Monteiro, Giovanna Merrelho; da Costa, Luciana Veloso; Dos Santos, Milena Cristina Silva; Dos Reis, Cristhiane Moura Falavina; Braga, Lygia Maria Paulo da Silva; Forsythe, Stephen James; Villas Bôas, Maria Helena Simões; Brandão, Marcelo Luiz Lima.
Afiliación
  • de Miranda RVDSL; Laboratory of Microbiological Control, Bio-Manguinhos/Fiocruz, CEP 21040-900, Rio de Janeiro, Brazil.
  • Monteiro GM; Laboratory of Microbiology of Food and Sanitizes, INCQS/Fiocruz, CEP 21040-900, Rio de Janeiro, Brazil.
  • da Costa LV; Laboratory of Microbiological Control, Bio-Manguinhos/Fiocruz, CEP 21040-900, Rio de Janeiro, Brazil.
  • Dos Santos MCS; Laboratory of Microbiological Control, Bio-Manguinhos/Fiocruz, CEP 21040-900, Rio de Janeiro, Brazil.
  • Dos Reis CMF; Laboratory of Microbiological Control, Bio-Manguinhos/Fiocruz, CEP 21040-900, Rio de Janeiro, Brazil.
  • Braga LMPDS; Laboratory of Microbiological Control, Bio-Manguinhos/Fiocruz, CEP 21040-900, Rio de Janeiro, Brazil.
  • Forsythe SJ; Laboratory of Microbiological Control, Bio-Manguinhos/Fiocruz, CEP 21040-900, Rio de Janeiro, Brazil.
  • Villas Bôas MHS; Foodmicrobe.com, Adams Hill, Keyworth, Nottinghamshire NG12 5GY, United Kingdom.
  • Brandão MLL; Laboratory of Microbiology of Food and Sanitizes, INCQS/Fiocruz, CEP 21040-900, Rio de Janeiro, Brazil.
J Appl Microbiol ; 134(10)2023 Oct 04.
Article en En | MEDLINE | ID: mdl-37838475
AIMS: Evaluate methods for identification and typing of Stenotrophomonas maltophilia isolated from a pharmaceutical facility. METHODS AND RESULTS: From 270 S. maltophilia strains identified by VITEK®2, 40 were selected and submitted to MALDI TOF-MS, 16S and 23S rRNA gene analysis, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and an antimicrobial susceptibility profile. 16S rRNA sequencing was able to identify 39 (97.5%) strains as Stenotrophomonas spp. and one (2.5%) as Luteimonas huabeiensis. MALDI TOF-MS identified 37 (92.5%) strains as S. maltophilia, and three (7.5%) were not identified. PCR targeting 23S rRNA yielded a positive result for 39 (97.5%) strains. However, after sequencing, two strains were identified as Stenotrophomonas rhizophila, showing false-positive results. The confirmed S. maltophilia strains (n = 37) showed 35 distinct ERIC-PCR profiles and exhibited sensitivity to minocycline and levofloxacin, and six (16.3%) showed intermediate resistance to sulfamethoxazole-trimethoprim. CONCLUSION: Matrix-assisted laser desorption lonization-time of flight mass spectrometry (MALDI-TOF MS) was a satisfactory methodology for the identification of S. maltophilia, but expansion of the database is necessary for the identification of other species. 16S rDNA sequencing showed low resolution for Stenotrophomonas species differentiation. PCR targeting 23S rRNA could not differentiate S. maltophilia from S. rhizophila. ERIC-PCR was shown to be a useful tool for the microbial source tracking of S. maltophilia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Bacterias Gramnegativas / Stenotrophomonas maltophilia Límite: Humans Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Bacterias Gramnegativas / Stenotrophomonas maltophilia Límite: Humans Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido