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Exosomal mRNAs for Angiogenic-Osteogenic Coupled Bone Repair.
Ma, Yifan; Sun, Lili; Zhang, Jingjing; Chiang, Chi-Ling; Pan, Junjie; Wang, Xinyu; Kwak, Kwang Joo; Li, Hong; Zhao, Renliang; Rima, Xilal Y; Zhang, Chi; Zhang, Anan; Liu, Yutong; He, Zirui; Hansford, Derek; Reategui, Eduardo; Liu, Changsheng; Lee, Andrew S; Yuan, Yuan; Lee, Ly James.
Afiliación
  • Ma Y; Department of Biomedical Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Sun L; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Zhang J; Key Laboratory for Ultrafine Materials of Ministry of Education and Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, 200237, Shanghai, P. R. China.
  • Chiang CL; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Pan J; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Wang X; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Kwak KJ; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Li H; Spot Biosystems Ltd., Palo Alto, 94305, United States.
  • Zhao R; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Rima XY; Department of Orthopedic Surgery and Shanghai Institute of Microsurgery on Extremities, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 200233, Shanghai, China.
  • Zhang C; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Zhang A; College of Pharmacy, The Ohio State University, Columbus, OH, 43210, USA.
  • Liu Y; Key Laboratory for Ultrafine Materials of Ministry of Education and Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, 200237, Shanghai, P. R. China.
  • He Z; Key Laboratory for Ultrafine Materials of Ministry of Education and Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, 200237, Shanghai, P. R. China.
  • Hansford D; Key Laboratory for Ultrafine Materials of Ministry of Education and Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, 200237, Shanghai, P. R. China.
  • Reategui E; Department of Biomedical Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Liu C; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
  • Lee AS; Key Laboratory for Ultrafine Materials of Ministry of Education and Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, 200237, Shanghai, P. R. China.
  • Yuan Y; School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China.
  • Lee LJ; Institute for Cancer Research, Shenzhen Bay Laboratory, 518055, Shenzhen, China.
Adv Sci (Weinh) ; 10(33): e2302622, 2023 11.
Article en En | MEDLINE | ID: mdl-37847907
ABSTRACT
Regenerative medicine in tissue engineering often relies on stem cells and specific growth factors at a supraphysiological dose. These approaches are costly and may cause severe side effects. Herein, therapeutic small extracellular vesicles (t-sEVs) endogenously loaded with a cocktail of human vascular endothelial growth factor A (VEGF-A) and human bone morphogenetic protein 2 (BMP-2) mRNAs within a customized injectable PEGylated poly (glycerol sebacate) acrylate (PEGS-A) hydrogel for bone regeneration in rats with challenging femur critical-size defects are introduced. Abundant t-sEVs are produced by a facile cellular nanoelectroporation system based on a commercially available track-etched membrane (TM-nanoEP) to deliver plasmid DNAs to human adipose-derived mesenchymal stem cells (hAdMSCs). Upregulated microRNAs associated with the therapeutic mRNAs are enriched in t-sEVs for enhanced angiogenic-osteogenic regeneration. Localized and controlled release of t-sEVs within the PEGS-A hydrogel leads to the retention of therapeutics in the defect site for highly efficient bone regeneration with minimal low accumulation in other organs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Factor A de Crecimiento Endotelial Vascular Límite: Animals / Humans Idioma: En Revista: Adv Sci (Weinh) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Factor A de Crecimiento Endotelial Vascular Límite: Animals / Humans Idioma: En Revista: Adv Sci (Weinh) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos