NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera.
Proc SPIE Int Soc Opt Eng
; 123842023.
Article
en En
| MEDLINE
| ID: mdl-37869412
ABSTRACT
SwissSPAD3 is the latest of a family of widefield time-gated SPAD imagers developed for fluorescence lifetime imaging (FLI) applications. Its distinctive features are (i) the ability to define shorter gates than its predecessors (width W < 1 ns), (ii) support for laser repetition rates up to at least 80 MHz and (iii) a dual-gate architecture providing an effective duty cycle of 100%. We present widefield macroscopic FLI measurements of short lifetime NIR dyes, analyzed using the phasor approach. The results are compared with those previously obtained with SwissSPAD2 and to theoretical predictions.
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01-internacional
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MEDLINE
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En
Revista:
Proc SPIE Int Soc Opt Eng
Año:
2023
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Article