Vitrified before and after genome editing via electroporation.
Cryo Letters
; 44(2): 118-122, 2023.
Article
en En
| MEDLINE
| ID: mdl-37883162
ABSTRACT
BACKGROUND:
Cryopreservation of bovine zygotes allows for a flexible schedule of genome editing via electroporation. However, vitrification-induced cell membrane damage may not only affect embryonic development but also genome mutation.OBJECTIVE:
To investigate the effects of vitrification of zygotes before and after electroporation treatments on the development and genome mutation of bovine presumptive zygotes. MATERIALS ANDMETHODS:
In vitro-derived bovine zygotes were electroporated with the CRISPR/Cas9 system immediately (Vitrified-EP) or 2 h after incubation (Vitrified-2h-EP) following vitrification and warming, or electroporated before vitrification (EP-vitrified).RESULTS:
The development rates of vitrified-warmed zygotes were significantly lower (p < 0.05) than those of control zygotes that were not vitrified. Moreover, no differences were observed in the mutation rates and mutation efficiency of the blastocysts resulting from electroporated zygotes, irrespective of the timing of electroporation treatment.CONCLUSION:
Our results suggest that vitrification before and after electroporation treatments does not affect the genome editing of zygotes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Criopreservación
/
Edición Génica
Límite:
Animals
Idioma:
En
Revista:
Cryo Letters
Asunto de la revista:
BIOLOGIA
/
QUIMICA
Año:
2023
Tipo del documento:
Article
País de afiliación:
Japón