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Integrated Meta-Omics Analysis Unveils the Pathways Modulating Tumorigenesis and Proliferation in High-Grade Meningioma.
Biswas, Deeptarup; Halder, Ankit; Barpanda, Abhilash; Ghosh, Susmita; Chauhan, Aparna; Bhat, Lipika; Epari, Sridhar; Shetty, Prakash; Moiyadi, Aliasgar; Ball, Graham Roy; Srivastava, Sanjeeva.
Afiliación
  • Biswas D; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
  • Halder A; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
  • Barpanda A; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
  • Ghosh S; Leibniz-Institut für Analytische Wissenschaften-ISAS, 44227 Dortmund, Germany.
  • Chauhan A; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
  • Bhat L; Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS Deemed-to-be University, Mumbai 400056, India.
  • Epari S; Department of Pathology, Tata Memorial Centre, Mumbai 400012, India.
  • Shetty P; Department of Neurosurgery, Tata Memorial Centre, Mumbai 400012, India.
  • Moiyadi A; Department of Neurosurgery, Tata Memorial Centre, Mumbai 400012, India.
  • Ball GR; Medical Technology Research Centre, Anglia Ruskin University, East Rd., Cambridge CB1 1PT, UK.
  • Srivastava S; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Cells ; 12(20)2023 10 18.
Article en En | MEDLINE | ID: mdl-37887327
Meningioma, a primary brain tumor, is commonly encountered and accounts for 39% of overall CNS tumors. Despite significant progress in clinical research, conventional surgical and clinical interventions remain the primary treatment options for meningioma. Several proteomics and transcriptomics studies have identified potential markers and altered biological pathways; however, comprehensive exploration and data integration can help to achieve an in-depth understanding of the altered pathobiology. This study applied integrated meta-analysis strategies to proteomic and transcriptomic datasets comprising 48 tissue samples, identifying around 1832 common genes/proteins to explore the underlying mechanism in high-grade meningioma tumorigenesis. The in silico pathway analysis indicated the roles of extracellular matrix organization (EMO) and integrin binding cascades in regulating the apoptosis, angiogenesis, and proliferation responsible for the pathobiology. Subsequently, the expression of pathway components was validated in an independent cohort of 32 fresh frozen tissue samples using multiple reaction monitoring (MRM), confirming their expression in high-grade meningioma. Furthermore, proteome-level changes in EMO and integrin cell surface interactions were investigated in a high-grade meningioma (IOMM-Lee) cell line by inhibiting integrin-linked kinase (ILK). Inhibition of ILK by administrating Cpd22 demonstrated an anti-proliferative effect, inducing apoptosis and downregulating proteins associated with proliferation and metastasis, which provides mechanistic insight into the disease pathophysiology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Meníngeas / Meningioma Tipo de estudio: Systematic_reviews Límite: Humans Idioma: En Revista: Cells Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Meníngeas / Meningioma Tipo de estudio: Systematic_reviews Límite: Humans Idioma: En Revista: Cells Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Suiza