COMPARATIVE STUDY OF BIOTOLERANCE CHARACTERISTICS OF DIFFERENT GELS COMPOSED OF BENZYDAMINE AND FLAVONOIDS THAT WERE DEVELOPED FOR TREATMENT OF PERIODONTAL DISEASES IN ORTHODONTIC PATIENTS.

OBJECTIVE: The aim: Different gels composed of benzydamine and flavonoids that were developed for treatment of periodontal diseases in the orthodontic patients will be compared regarding their effects on survival of mammalian cells of various tissue origin and their DNA intactness. PATIENTS AND METHODS: Materials and methods: Effect of different variants of patented gel composition «Benzidaflaziverdine¼ including a gel base and «Proteflazid®¼ containing flavonoids and benzydamine hydrochloride in powder form («T-Sept®¼) towards survival (MTT) of murine BALB-3T3 fibroblasts, J774.2 macrophages, human HaCaT keratinocytes was studied. Their effect on nativity of DNA of J774.2 macrophages was evaluated using DNA-comet assay. RESULTS: Results: Three gel compositions were used. Sample 1 was prepared on gel basis including benzydamine in liquid form and demonstrated inhibitory effect towards pseudonormal murine BALB-3T3 fibroblasts and murine J774.2 macrophages, however, normal human НаСаТ keratinocytes were resistant to its action. Sample 2 included BH in powder form and it did not affect significantly НаСаТ keratinocytes аnd BALB-3T3 fibroblasts, but it suppressed J774.2 macrophages. Sample 3 («Benzidaflaziverdine¼) was developed and patented by us as a gel composed of benzydamine in powder form and flavonoid drops «Proteflazid®¼. It did not suppress tested mammalian cells and was not genotoxic (measured as % of DNA in comet tail and Olive Tail Moment) for murine J774.2 macrophages. CONCLUSION: Conclusions: Inclusion of flavonoids in gel composition «Benzidaflaziverdine¼ blocked cytotoxic and genotoxic actions of benzydamine. Developed gel com¬position might be efficient in clinical periodontology, in particular, for treatment of periodontal diseases in orthodontic patients.