Your browser doesn't support javascript.
loading
CAMSAP1 role in orchestrating structure and dynamics of manchette microtubule minus-ends impacts male fertility during spermiogenesis.
Hu, Weichang; Zhang, Rui; Xu, Honglin; Li, Yuejia; Yang, Xiaojuan; Zhou, Zhengrong; Huang, Xiahe; Wang, Yingchun; Ji, Wei; Gao, Fei; Meng, Wenxiang.
Afiliación
  • Hu W; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 10019, China.
  • Zhang R; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • Xu H; National Laboratory of Biomacromolecules, Chinese Academy of Sciences Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • Li Y; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 10019, China.
  • Yang X; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 10019, China.
  • Zhou Z; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • Huang X; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 10019, China.
  • Wang Y; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • Ji W; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 10019, China.
  • Gao F; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • Meng W; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 10019, China.
Proc Natl Acad Sci U S A ; 120(45): e2313787120, 2023 Nov 07.
Article en En | MEDLINE | ID: mdl-37903275
The manchette is a crucial transient structure involved in sperm development, with its composition and regulation still not fully understood. This study focused on investigating the roles of CAMSAP1 and CAMSAP2, microtubule (MT) minus-end binding proteins, in regulating manchette MTs, spermiogenesis, and male fertility. The loss of CAMSAP1, but not CAMSAP2, disrupts the well-orchestrated process of spermiogenesis, leading to abnormal manchette elongation and delayed removal, resulting in deformed sperm nuclei and tails resembling oligoasthenozoospermia symptoms. We investigated the underlying molecular mechanisms by purifying manchette assemblies and comparing them through proteomic analysis, and results showed that the absence of CAMSAP1 disrupted the proper localization of key proteins (CEP170 and KIF2A) at the manchette minus end, compromising its structural integrity and hindering MT depolymerization. These findings highlight the significance of maintaining homeostasis in manchette MT minus-ends for shaping manchette morphology during late spermiogenesis, offering insights into the molecular mechanisms underlying infertility and sperm abnormalities.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Proteómica Límite: Humans / Male Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Proteómica Límite: Humans / Male Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos