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Exploring the antimicrobial potential of isoniazid loaded Cu-based metal-organic frameworks as a novel strategy for effective killing of Mycobacterium tuberculosis.
Kumar, Pawan; Behera, Ananyaashree; Tiwari, Pranav; Karthik, Sibi; Biswas, Mainak; Sonawane, Avinash; Mobin, Shaikh M.
Afiliación
  • Kumar P; Department of Chemistry, Indian Institute of Technology, Indore, Simrol, Madhya Pradesh, India.
  • Behera A; School of Biotechnology, KIIT Deemed to be University, Bhubaneswar, Odisha, India. xray@iiti.ac.in.
  • Tiwari P; Department of Chemistry, Indian Institute of Technology, Indore, Simrol, Madhya Pradesh, India.
  • Karthik S; Department of Biosciences and Biomedical Engineering, Indian Institute of Technology, Indore, Simrol, Madhya Pradesh, India.
  • Biswas M; School of Biotechnology, KIIT Deemed to be University, Bhubaneswar, Odisha, India. xray@iiti.ac.in.
  • Sonawane A; School of Biotechnology, KIIT Deemed to be University, Bhubaneswar, Odisha, India. xray@iiti.ac.in.
  • Mobin SM; Department of Biosciences and Biomedical Engineering, Indian Institute of Technology, Indore, Simrol, Madhya Pradesh, India.
J Mater Chem B ; 11(45): 10929-10940, 2023 11 22.
Article en En | MEDLINE | ID: mdl-37937634
Tuberculosis (TB) remains one of the most infectious pathogens with the highest human mortality and morbidity. Biofilm formation during Mycobacterium tuberculosis (Mtb) infection is responsible for bacterial growth, communication, and, most essentially, increased resistance/tolerance to antibiotics leading to higher bacterial persistence. Thus, biofilm growth is presently considered a key virulence factor in the case of chronic disease. Metal-Organic Frameworks (MOFs) have recently emerged as a highly efficient system to improve existing antibiotics' therapeutic efficacy and reduce adverse effects. In this regard, we have synthesized Cu-MOF (IITI-3) using a solvothermal approach. IITI-3 was well characterized by various spectroscopic techniques. Herein, IITI-3 was first encapsulated with isoniazid (INH) to form INH@IITI-3 with 10 wt% loading within 1 hour. INH@IITI-3 was well characterized by PXRD, TGA, FTIR, and BET surface area analysis. Furthermore, the drug release kinetics studies of INH@IITI-3 have been performed at pH 5.8 and 7.4 to mimic the small intestine and blood pH, respectively. The results show that drug release follows first-order kinetics. Furthermore, the antimycobacterial activity of INH@IITI-3 demonstrated significant bacterial killing and altered the structural morphology of the bacteria. Moreover, INH@IITI-3 was able to inhibit the mycobacterial biofilm formation upon treatment and showed less cytotoxicity toward the murine RAW264.7 macrophages. Thus, this work significantly opens up new possibilities for the applications of INH@IITI-3 in biofilm infections in Mtb and further contributes to TB therapeutics.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Estructuras Metalorgánicas / Mycobacterium tuberculosis Límite: Animals / Humans Idioma: En Revista: J Mater Chem B Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Estructuras Metalorgánicas / Mycobacterium tuberculosis Límite: Animals / Humans Idioma: En Revista: J Mater Chem B Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido